Mouse Anti-COL15A1 Recombinant Antibody (CBFYC-2035) (V2LY-1206-LY599)

Name: Mouse Anti-COL15A1 Recombinant Antibody (CBFYC-2035)
SKU: V2LY-1206-LY599
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-2035

Application

WB, IF

Immunogen

Full length human recombinant protein of human COL15A1 produced in HEK293T cell.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:4,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.3, 1% BSA, 50% Glycerol

Preservative

0.02% sodium azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Collagen Type XV Alpha 1 Chain

Entrez Gene ID

1306

UniProt ID

P39059

Function

Structural protein that stabilizes microvessels and muscle cells, both in heart and in skeletal muscle.

Restin potently inhibits angiogenesis.

Biological Process

Angiogenesis Source: GO_Central
Cell adhesion Source: UniProtKB-KW
Collagen catabolic process Source: Reactome
Collagen fibril organization Source: Reactome
Endothelial cell morphogenesis Source: GO_Central
Extracellular matrix organization Source: GO_Central
Signal transduction Source: UniProtKB

Cellular Location

Extracellular matrix

PTM

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-glycosylated; with core 1 or possibly core 8 glycans. An N-terminal peptide contains chondroitin sulfate.

Wu, J., Ren, W., Zheng, Z., Huang, Z., Liang, T., Li, F., ... & Guo, L. (2020). Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 by targeting COL15A1. Molecular Medicine Reports, 22(3), 1737-1746.

Shevela, A. I., Gavrilov, K. A., Plotnikova, E. Y., Sevostianova, K. S., Filipenko, M. L., & Smetanina, M. A. (2020). Altered expression of the extracellular matrix related genes COL15A1, CHRDL2, EFEMP1, and TIMP1 in Varicose Veins. European Journal of Vascular and Endovascular Surgery, 60(4), e75-e76.

Bretaud, S., Guillon, E., Karppinen, S. M., Pihlajaniemi, T., & Ruggiero, F. (2020). Collagen XV, a multifaceted multiplexin present across tissues and species. Matrix Biology Plus, 6, 100023.

Durgin, B. G., Cherepanova, O. A., Gomez, D., Karaoli, T., Alencar, G. F., Butcher, J. T., ... & Connelly, J. J. (2017). Smooth muscle cell-specific deletion of Col15a1 unexpectedly leads to impaired development of advanced atherosclerotic lesions. American Journal of Physiology-Heart and Circulatory Physiology, 312(5), H943-H958.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Human COL15A1 ELISA Kit (CAT#: V2LY-0626-LY3545)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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