Human COL15A1 ELISA Kit (V2LY-0626-LY3545)

Name: Human COL15A1 ELISA Kit
SKU: V2LY-0626-LY3545
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.046 ng/mL

Detection Range

0.1-40 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

177.4 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Collagen Type XV Alpha 1 Chain

Function

Structural protein that stabilizes microvessels and muscle cells, both in heart and in skeletal muscle.

Restin potently inhibits angiogenesis.

Biological Process

Angiogenesis Source: GO_Central
Cell adhesion Source: UniProtKB-KW
Collagen catabolic process Source: Reactome
Collagen fibril organization Source: Reactome
Endothelial cell morphogenesis Source: GO_Central
Extracellular matrix organization Source: GO_Central
Signal transduction Source: UniProtKB

Cellular Location

Extracellular matrix

PTM

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
O-glycosylated; with core 1 or possibly core 8 glycans. An N-terminal peptide contains chondroitin sulfate.

Wu, J., Ren, W., Zheng, Z., Huang, Z., Liang, T., Li, F., ... & Guo, L. (2020). Mmu_circ_003795 regulates osteoblast differentiation and mineralization in MC3T3‑E1 and MDPC23 by targeting COL15A1. Molecular Medicine Reports, 22(3), 1737-1746.

Shevela, A. I., Gavrilov, K. A., Plotnikova, E. Y., Sevostianova, K. S., Filipenko, M. L., & Smetanina, M. A. (2020). Altered expression of the extracellular matrix related genes COL15A1, CHRDL2, EFEMP1, and TIMP1 in Varicose Veins. European Journal of Vascular and Endovascular Surgery, 60(4), e75-e76.

Bretaud, S., Guillon, E., Karppinen, S. M., Pihlajaniemi, T., & Ruggiero, F. (2020). Collagen XV, a multifaceted multiplexin present across tissues and species. Matrix Biology Plus, 6, 100023.

Durgin, B. G., Cherepanova, O. A., Gomez, D., Karaoli, T., Alencar, G. F., Butcher, J. T., ... & Connelly, J. J. (2017). Smooth muscle cell-specific deletion of Col15a1 unexpectedly leads to impaired development of advanced atherosclerotic lesions. American Journal of Physiology-Heart and Circulatory Physiology, 312(5), H943-H958.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-COL15A1 Recombinant Antibody (CBFYC-2035) (CAT#: V2LY-1206-LY599)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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