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Mouse Anti-COX5B Recombinant Antibody (CBFYC-2125) (V2LY-1206-LY874)


See all COX5B antibodies
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYC-2125
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 35-129 mapping at the C-terminus of human COX5b.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Cytochrome C Oxidase Subunit 5B
Entrez Gene ID
Human1329
Mouse12859
Rat94194
UniProt ID
HumanP10606
MouseP19536
RatP12075
Function
Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Biological Process
Mitochondrial electron transport, cytochrome c to oxygen Source: GO_Central
Respiratory gaseous exchange by respiratory system Source: ProtInc
Cellular Location
Mitochondrion inner membrane
More Infomation

Chu, Y. D., Lim, S. N., Yeh, C. T., & Lin, W. R. (2022). COX5B-Mediated Bioenergetic Alterations Modulate Cell Growth and Anticancer Drug Susceptibility by Orchestrating Claudin-2 Expression in Colorectal Cancers. Biomedicines, 10(1), 60.

Shahali, A., Aghaei, N. S. G., Mosharraf, A., Sharestani, S., & Madani, A. (2022). Study of the expression rates of RORA and COX5b genes amongst MS patients compared with healthy individuals as an emerging diagnostic biomarker. Academic Journal of Health Sciencies: Medicina balear, 37(1), 161-165.

Chu, Y. D., Lin, W. R., Lin, Y. H., Kuo, W. H., Tseng, C. J., Lim, S. N., ... & Yeh, C. T. (2020). COX5B-mediated bioenergetic alteration regulates tumor growth and migration by modulating AMPK-UHMK1-ERK cascade in hepatoma. Cancers, 12(6), 1646.

Stein, J., Tenbrock, J., Kristiansen, G., Müller, S. C., & Ellinger, J. (2019). Systematic expression analysis of the mitochondrial respiratory chain protein subunits identifies COX5B as a prognostic marker in clear cell renal cell carcinoma. International Journal of Urology, 26(9), 910-916.

Hinkelbein, J., Böhm, L., Braunecker, S., Adler, C., De Robertis, E., & Cirillo, F. (2017). Decreased tissue COX5B expression and mitochondrial dysfunction during sepsis-induced kidney injury in rats. Oxidative medicine and cellular longevity, 2017.

Hu, T., & Xi, J. (2017). Identification of COX5B as a novel biomarker in high-grade glioma patients. OncoTargets and therapy, 10, 5463.

Gao, S. P., Sun, H. F., Fu, W. Y., Li, L. D., Zhao, Y., Chen, M. T., & Jin, W. (2017). High expression of COX5B is associated with poor prognosis in breast cancer. Future Oncology, 13(19), 1711-1719.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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