Human Recombinant COX5B protein, His Tag (V2LY-0526-LY3347)

Name: Human Recombinant COX5B protein, His Tag
SKU: V2LY-0526-LY3347
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant COX5B protein, His Tag consist of Amino Acid: 32-129 and predicts a molecular mass of 12.5 kDa.

Molecule Mass

12.5 kDa

Sequence

Amino Acid: 32-129

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Cytochrome C Oxidase Subunit 5B

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

Biological Process

Mitochondrial electron transport, cytochrome c to oxygen Source: GO_Central
Respiratory gaseous exchange by respiratory system Source: ProtInc

Cellular Location

Mitochondrion inner membrane

Chu, Y. D., Lim, S. N., Yeh, C. T., & Lin, W. R. (2022). COX5B-Mediated Bioenergetic Alterations Modulate Cell Growth and Anticancer Drug Susceptibility by Orchestrating Claudin-2 Expression in Colorectal Cancers. Biomedicines, 10(1), 60.

Shahali, A., Aghaei, N. S. G., Mosharraf, A., Sharestani, S., & Madani, A. (2022). Study of the expression rates of RORA and COX5b genes amongst MS patients compared with healthy individuals as an emerging diagnostic biomarker. Academic Journal of Health Sciencies: Medicina balear, 37(1), 161-165.

Chu, Y. D., Lin, W. R., Lin, Y. H., Kuo, W. H., Tseng, C. J., Lim, S. N., ... & Yeh, C. T. (2020). COX5B-mediated bioenergetic alteration regulates tumor growth and migration by modulating AMPK-UHMK1-ERK cascade in hepatoma. Cancers, 12(6), 1646.

Stein, J., Tenbrock, J., Kristiansen, G., Müller, S. C., & Ellinger, J. (2019). Systematic expression analysis of the mitochondrial respiratory chain protein subunits identifies COX5B as a prognostic marker in clear cell renal cell carcinoma. International Journal of Urology, 26(9), 910-916.

Hinkelbein, J., Böhm, L., Braunecker, S., Adler, C., De Robertis, E., & Cirillo, F. (2017). Decreased tissue COX5B expression and mitochondrial dysfunction during sepsis-induced kidney injury in rats. Oxidative medicine and cellular longevity, 2017.

Hu, T., & Xi, J. (2017). Identification of COX5B as a novel biomarker in high-grade glioma patients. OncoTargets and therapy, 10, 5463.

Gao, S. P., Sun, H. F., Fu, W. Y., Li, L. D., Zhao, Y., Chen, M. T., & Jin, W. (2017). High expression of COX5B is associated with poor prognosis in breast cancer. Future Oncology, 13(19), 1711-1719.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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