Mouse Anti-CRLF2 Recombinant Antibody (CBYJT-1151) (V2LY-1206-LY1196)

Name: Mouse Anti-CRLF2 Recombinant Antibody (CBYJT-1151)
SKU: V2LY-1206-LY1196
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJT-1151

Application

FC, WB

Immunogen

Recombinant human TSLPR Gly25-Lys231.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	0.5 µg/ml
FC	0.25 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cytokine Receptor Like Factor 2

Entrez Gene ID

64109

UniProt ID

Q9HC73

Function

Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity).

Implicated in the development of the hematopoietic system.

Biological Process

Cytokine-mediated signaling pathway Source: UniProtKB
Interleukin-7-mediated signaling pathway Source: Reactome
Oncostatin-M-mediated signaling pathway Source: GOC
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of interleukin-5 production Source: UniProtKB
Positive regulation of mast cell activation Source: UniProtKB
Positive regulation of receptor signaling pathway via STAT Source: UniProtKB

Cellular Location

Isoform 1: Cell membrane
Isoform 2: Secreted

Topology

Extracellular: 23-231
Helical: 232-252
Cytoplasmic: 253-371

Rasekh, E. O., Atef, A. M., Khalil, M., Ebeid, E., Madney, Y., & Hamdy, N. (2021). Characterization of CRLF2 Expression in Pediatric B-Cell Precursor Acute Lymphoblastic Leukemia. Clinical Laboratory, 67(1).

Mohamed, S. M., Wohlmann, A., Schofield, P., Sia, K. C., McCalmont, H., Savvides, S. N., ... & Lock, R. B. (2021). A recombinant antibody fragment directed to the thymic stromal lymphopoietin receptor (CRLF2) efficiently targets pediatric Philadelphia chromosome-like acute lymphoblastic leukemia. International Journal of Biological Macromolecules, 190, 214-223.

Moreno-Lorenzana, D., Juárez-Velázquez, R., Martínez-Anaya, D., & Péez-Vera, P. (2020). CRLF2 (cytokine receptor like factor 2). Atlas of Genetics and Cytogenetics in Oncology and Haematology.

Meyer, L. K., Delgado-Martin, C., Maude, S. L., Shannon, K. M., Teachey, D. T., & Hermiston, M. L. (2019). CRLF2 rearrangement in Ph-like acute lymphoblastic leukemia predicts relative glucocorticoid resistance that is overcome with MEK or Akt inhibition. PloS one, 14(7), e0220026.

Zhang, Q., Shi, C., Han, L., Jain, N., Roberts, K. G., Ma, H., ... & Konopleva, M. (2018). Inhibition of mTORC1/C2 signaling improves anti-leukemia efficacy of JAK/STAT blockade in CRLF2 rearranged and/or JAK driven Philadelphia chromosome–like acute B-cell lymphoblastic leukemia. Oncotarget, 9(8), 8027.

Pastorczak, A., Sedek, L., Braun, M., Madzio, J., Sonsala, A., Twardoch, M., ... & Młynarski, W. (2018). Surface expression of Cytokine Receptor-Like Factor 2 increases risk of relapse in pediatric acute lymphoblastic leukemia patients harboring IKZF1 deletions. Oncotarget, 9(40), 25971.

Mangaonkar, A., Patnaik, M. M., Kaiser-Rogers, K., Rao, K. W., Halling, K., Oliver, G., ... & Van Dyke, D. L. (2017). Cytokine Receptor-like Factor 2 (CRLF2) Overexpression in Myeloid Malignancies Secondary to Multiple Isodicentric Y Chromosomes: A Novel Cytogenetic Entity?. Blood, 130, 1702.

Konoplev, S., Lu, X., Konopleva, M., Jain, N., Ouyang, J., Goswami, M., ... & Wang, S. A. (2017). CRLF2-Positive B-Cell Acute Lymphoblastic Leukemia in Adult PatientsA Single-Institution Experience. American journal of clinical pathology, 147(4), 357-363.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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