Mouse Anti-CXCL5 Recombinant Antibody (CBXC-1116) (V2LY-0125-LY709)

Name: Mouse Anti-CXCL5 Recombinant Antibody (CBXC-1116)
SKU: V2LY-0125-LY709
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXC-1116

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 85-114 at the C-terminus of human ENA-78.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

C-X-C Motif Chemokine Ligand 5

Entrez Gene ID

6374

UniProt ID

P42830

Function

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

Biological Process

Antimicrobial humoral immune response mediated by antimicrobial peptide Source: GO_Central
Cell-cell signaling Source: ProtInc
Cellular response to lipopolysaccharide Source: GO_Central
Chemokine-mediated signaling pathway Source: GO_Central
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: Reactome
Inflammatory response Source: GO_Central
Neutrophil chemotaxis Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Signal transduction Source: ProtInc

Cellular Location

Secreted

PTM

N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Kalchiem-Dekel, O., Yao, X., Barochia, A. V., Kaler, M., Figueroa, D. M., Karkowsky, W. B., ... & Levine, S. J. (2020). Apolipoprotein E signals via TLR4 to induce CXCL5 secretion by asthmatic airway epithelial cells. American Journal of Respiratory Cell and Molecular Biology, 63(2), 185-197.

Bai, L., Yao, N., Qiao, G., Wu, L., & Ma, X. (2020). CXCL5 contributes to the tumorigenicity of cervical cancer and is post-transcriptionally regulated by miR-577. International Journal of Clinical and Experimental Pathology, 13(12), 2984.

Zhang, W., Wang, H., Sun, M., Deng, X., Wu, X., Ma, Y., ... & Miao, L. (2020). CXCL5/CXCR2 axis in tumor microenvironment as potential diagnostic biomarker and therapeutic target. Cancer Communications, 40(2-3), 69-80.

Cui, D., Zhao, Y., & Xu, J. (2019). Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38 pathways. Cancer biology & therapy, 20(5), 608-616.

Wang, C. Y., Shang, M., Zhou, C. L., Feng, L. Z., Zhou, Q. S., & Hu, K. (2019). Mechanism of Cxc chemokine ligand 5 (CXCL5)/Cxc chemokine receptor 2 (CXCR2) bio-axis in mice with acute respiratory distress syndrome. Medical science monitor: international medical journal of experimental and clinical research, 25, 5299.

Lim, W. K., Chai, X., Ghosh, S., Ray, D., Wang, M., Rasheed, S. A. K., & Casey, P. J. (2019). Gα-13 induces CXC motif chemokine ligand 5 expression in prostate cancer cells by transactivating NF-κB. Journal of Biological Chemistry, 294(48), 18192-18206.

Wang, C., Li, A., Yang, S., Qiao, R., Zhu, X., & Zhang, J. (2018). CXCL5 promotes mitomycin C resistance in non-muscle invasive bladder cancer by activating EMT and NF-κB pathway. Biochemical and biophysical research communications, 498(4), 862-868.

O'Sullivan, S. A., O'Sullivan, C., Healy, L. M., Dev, K. K., & Sheridan, G. K. (2018). Sphingosine 1‐phosphate receptors regulate TLR 4‐induced CXCL 5 release from astrocytes and microglia. Journal of neurochemistry, 144(6), 736-747.

Zhao, Y., Zhang, X., Zhao, H., Wang, J., & Zhang, Q. (2018). CXCL5 secreted from adipose tissue‑derived stem cells promotes cancer cell proliferation. Oncology Letters, 15(2), 1403-1410.

Smith, K. J., Boyer, J. A., Muku, G. E., Murray, I. A., Gowda, K., Desai, D., ... & Perdew, G. H. (2017). Editor’s highlight: Ah receptor activation potentiates neutrophil chemoattractant (CXC motif) ligand 5 expression in keratinocytes and skin. Toxicological Sciences, 160(1), 83-94.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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