Mouse Anti-DAP Recombinant Antibody (EG922) (V2LY-0125-LY1085)

Name: Mouse Anti-DAP Recombinant Antibody (EG922)
SKU: V2LY-0125-LY1085
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

EG922

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 11-45 near the N-terminus of human DAP-1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Death Associated Protein

Entrez Gene ID

Human	1611
Mouse	223453

UniProt ID

Human

P51397

Function

Negative regulator of autophagy. Involved in mediating interferon-gamma-induced cell death.

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
Apoptotic process Source: MGI
Apoptotic signaling pathway Source: UniProtKB
Autophagy Source: UniProtKB-KW
Cellular response to amino acid starvation Source: UniProtKB
Negative regulation of autophagy Source: UniProtKB
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB

PTM

Phosphorylated. Phosphorylation by MTOR inhibits the suppressive activity of DAP toward autophagy.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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