Mouse Anti-DCT Recombinant Antibody (CBYJT-1184) (V2LY-0125-LY1233)

Name: Mouse Anti-DCT Recombinant Antibody (CBYJT-1184)
SKU: V2LY-0125-LY1233
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DCT Recombinant Antibody (CBYJT-1184) (V2LY-0125-LY1233) in IF

Characterization of the primary culture of melanocytes from neonatal foreskin by detecting the expression of melanosomal glycoproteins TRP-1 (left) and TRP-2 (right) using immunofluorescence staining. ...View More

Alghamdi, K., Alehaideb, Z., Kumar, A., Al-Eidi, H., Alghamdi, S. S., Suliman, R., ... & Matou-Nasri, S. (2023). Stimulatory effects of Lycium shawii on human melanocyte proliferation, migration, and melanogenesis: In vitro and in silico studies. Frontiers in Pharmacology, 14, 1169812. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-DCT Recombinant Antibody (CBYJT-1184) (V2LY-0125-LY1233) in WB

Modulatory effects of the L. shawii extract on the protein expression levels of MITF, tyrosinase, TRP-1, and TRP-2 melanogenesis-related proteins using Western blot analysis. Representative Western blots showing the variation of melanogenesis-related protein expression levels in melanocytes after exposure to various concentrations (7.8-31.3 μg/mL) of the L. shawii extract. The bar graphs show the relative expression of each melanogenesis-related protein calculated as the ratio to the housekeeping protein α-tubulin, compared to the untreated cells. The results are presented as mean ± SD of three independent experiments. *p < 0.05 signify a statistically significant difference compared with the control. ...View More

Mouse Anti-DCT Recombinant Antibody (CBYJT-1184) (V2LY-0125-LY1233) in WB

Protein expression levels of TYR, TRP-1, TRP-2, and MITF were determined by Western blot following with acenocoumarol (10, 20 and 40 μM) treatment for 48 h in the presence of α-MSH (100 nM). ...View More

Han, H., & Hyun, C. (2023). Acenocoumarol, an anticoagulant drug, prevents melanogenesis in B16F10 melanoma cells. Pharmaceuticals, 16(4), 604. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-DCT Recombinant Antibody (CBYJT-1184) (V2LY-0125-LY1233) in Immunoblotting and IF

Expression of TYRP1, but not TYR or TYRP2, was substantially decreased in Mewo-Z5Z7-DKO cells. Immunoblotting (a) and immunofluorescence staining (b). ...View More

Wagatsuma, T., Suzuki, E., Shiotsu, M., Sogo, A., Nishito, Y., Ando, H., ... & Kambe, T. (2023). Pigmentation and TYRP1 expression are mediated by zinc through the early secretory pathway-resident ZNT proteins. Communications biology, 6(1), 403. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-DCT Recombinant Antibody (CBYJT-1184) (V2LY-0125-LY1233) in WB

DMI inhibits the expression of TYR, TRP-1, and TRP-2 in B16F10 cells. B16F10 cells (1.2 × 106 cells) were exposed to DMI at various times, and the protein expression of TYR, TRP-1 and TRP-2 was measured by Western blot analysis. The images of the films were analyzed by densitometry and calculated (n = 3). Representative images of the films are provided. ...View More

Yu, B. Y., Ngo, H. H., Choi, W. J., & Keum, Y. S. (2023). Dimethyl itaconate inhibits melanogenesis in B16F10 cells. Antioxidants, 12(3), 692. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJT-1184

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 41-190 of human TRP2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Dopachrome Tautomerase

Entrez Gene ID

Human	1638
Mouse	13190

UniProt ID

Human	P40126
Mouse	P29812

Function

Catalyzes the conversion of L-dopachrome into 5,6-dihydroxyindole-2-carboxylic acid (DHICA).

Biological Process

Cell development Source: Ensembl
Developmental pigmentation Source: GO_Central
Epidermis development Source: ProtInc
Melanin biosynthetic process Source: Reactome
Melanin biosynthetic process from tyrosine Source: UniProtKB
Positive regulation of neuroblast proliferation Source: GO_Central
Response to blue light Source: UniProtKB
Ventricular zone neuroblast division Source: GO_Central

Cellular Location

Melanosome membrane; Melanosome. Proper trafficking to melanosome is regulated by SGSM2, ANKRD27, RAB9A, RAB32 and RAB38.

Topology

Lumenal, melanosome: 24-472
Helical: 473-493
Cytoplasmic: 494-519

PTM

Glycosylated.

Volk, A. E., Hedergott, A., Preising, M., Rading, S., Fricke, J., Herkenrath, P., ... & Kubisch, C. (2021). Biallelic mutations in L-dopachrome tautomerase (DCT) cause infantile nystagmus and oculocutaneous albinism. Human Genetics, 140(8), 1157-1168.

Pennamen, P., Tingaud-Sequeira, A., Gazova, I., Keighren, M., McKie, L., Marlin, S., ... & Arveiler, B. (2021). Dopachrome tautomerase variants in patients with oculocutaneous albinism. Genetics in Medicine, 23(3), 479-487.

Rosani, U., Domeneghetti, S., Maso, L., Wegner, K. M., & Venier, P. (2019). An evolutionary perspective of dopachrome tautomerase enzymes in metazoans. Genes, 10(7), 495.

Salinas‑Santander, M., Trevino, V., de la Rosa‑Moreno, E., Verduzco‑Garza, B., Sánchez‑Domínguez, C. N., Cantú‑Salinas, C., ... & Ortiz‑López, R. (2018). CAPN3, DCT, MLANA and TYRP1 are overexpressed in skin of vitiligo vulgaris Mexican patients. Experimental and Therapeutic Medicine, 15(3), 2804-2811.

Dardente, H., & Lomet, D. (2018). Photoperiod and thyroid hormone regulate expression of l‐dopachrome tautomerase (Dct), a melanocyte stem‐cell marker, in tanycytes of the ovine hypothalamus. Journal of Neuroendocrinology, 30(9), e12640.

Sultana, H., Seo, D., Choi, N. R., Bhuiyan, M. S. A., Lee, S. H., Heo, K. N., & Lee, J. H. (2018). Identification of polymorphisms in MITF and DCT genes and their associations with plumage colors in Asian duck breeds. Asian-Australasian journal of animal sciences, 31(2), 180.

Aksoy, P., & Meneses, P. I. (2017). The role of DCT in HPV16 infection of HaCaTs. PLoS One, 12(1), e0170158.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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