Mouse Anti-DDR1 Recombinant Antibody (D0500) (V2LY-0425-LY1)

Name: Mouse Anti-DDR1 Recombinant Antibody (D0500)
SKU: V2LY-0425-LY1
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DDR1 Recombinant Antibody (D0500) (V2LY-0321-LY1-1) in IF

Left: Immunofluorescence of F-actin and DDR1 in HUVECs that were transfected with DDR1-siRNA or scrambled control siRNA (si-Scr) and subjected to PS or OS for 24h. HUVECs were all grown on gelatin-coated glass slide. Right: Quantification of alignment by measuring the orientation angle. n=9 images from 3 biological replicates. Scale bar: 50μm. ...View More

Liu, J., Zhao, C., Xiao, X., Li, A., Liu, Y., Zhao, J., ... & Zhou, J. (2023). Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation. Nature communications, 14(1), 6457.

Mouse Anti-DDR1 Recombinant Antibody (D0500) (V2LY-0321-LY1-2) in WB

Left: Co-IP between DDR1 and YWHAE in HUVECs cultured under the static condition or subjected to PS or OS for 3h. IP was performed with anti-DDR1 antibody. HC: Heavy chain. Right: quantification of YWHAE immunoprecipitated with DDR1. n=4 biological replicates. Data were expressed as the means±SEM and analyzed by Kruskal-Wallis test with Dunn's test. ...View More

Liu, J., Zhao, C., Xiao, X., Li, A., Liu, Y., Zhao, J., ... & Zhou, J. (2023). Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation. Nature communications, 14(1), 6457.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0500

Application

WB, ELISA, IP, IF

Immunogen

Amino acids 291-416 of human DDR1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Discoidin Domain Receptor Tyrosine Kinase 1

Entrez Gene ID

Human	780
Mouse	12305
Rat	25678
Monkey	714233

UniProt ID

Human	Q08345
Mouse	Q03146
Rat	Q63474
Monkey	F7BW77

Function

Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity).

Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.

Biological Process

Axon development Source: Ensembl
Branching involved in mammary gland duct morphogenesis Source: Ensembl
Cell adhesion Source: ProtInc
Collagen-activated tyrosine kinase receptor signaling pathway Source: UniProtKB
Ear development Source: Ensembl
Embryo implantation Source: Ensembl
Extracellular matrix organization Source: Reactome
Lactation Source: UniProtKB-KW
Mammary gland alveolus development Source: Ensembl
Multicellular organism development Source: GO_Central
Negative regulation of cell population proliferation Source: Ensembl
Neuron projection extension Source: Ensembl
Peptidyl-tyrosine autophosphorylation Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Protein autophosphorylation Source: UniProtKB
Regulation of cell growth Source: Ensembl
Regulation of cell-matrix adhesion Source: Ensembl
Regulation of extracellular matrix disassembly Source: UniProtKB
Smooth muscle cell-matrix adhesion Source: UniProtKB
Smooth muscle cell migration Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Wound healing, spreading of cells Source: UniProtKB

Cellular Location

Isoform 1&2&4: Cell membrane
Isoform 3: Secreted

Topology

Extracellular: 21-417
Helical: 418-438
Cytoplasmic: 439-913

PTM

Autophosphorylated in response to fibrillar collagen binding.
Glycosylation of Asn-211, but apparently not of Asn-260 or Asn-394, prevents autophosphorylation from occurring in the absence of collagen.

Zhang, X., Hu, Y., Pan, Y., Xiong, Y., Zhang, Y., Han, M., ... & Zhang, B. (2022). DDR1 promotes hepatocellular carcinoma metastasis through recruiting PSD4 to ARF6. Oncogene, 41(12), 1821-1834.

Chen, L. Y., Zhi, Z., Wang, L., Zhao, Y. Y., Deng, M., Liu, Y. H., ... & Li, J. M. (2019). NSD2 circular RNA promotes metastasis of colorectal cancer by targeting miR‐199b‐5p‐mediated DDR1 and JAG1 signalling. The Journal of Pathology, 248(1), 103-115.

Zhavoronkov, A., Ivanenkov, Y. A., Aliper, A., Veselov, M. S., Aladinskiy, V. A., Aladinskaya, A. V., ... & Aspuru-Guzik, A. (2019). Deep learning enables rapid identification of potent DDR1 kinase inhibitors. Nature biotechnology, 37(9), 1038-1040.

Vehlow, A., & Cordes, N. (2019). DDR1 (discoidin domain receptor tyrosine kinase 1) drives glioblastoma therapy resistance by modulating autophagy. Autophagy, 15(8), 1487-1488.

Moll, S., Desmoulière, A., Moeller, M. J., Pache, J. C., Badi, L., Arcadu, F., ... & Prunotto, M. (2019). DDR1 role in fibrosis and its pharmacological targeting. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(11), 118474.

Yeh, Y. C., Lin, H. H., & Tang, M. J. (2019). Dichotomy of the function of DDR1 in cells and disease progression. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1866(11), 118473.

Belfiore, A., Malaguarnera, R., Nicolosi, M. L., Lappano, R., Ragusa, M., Morrione, A., & Vella, V. (2018). A novel functional crosstalk between DDR1 and the IGF axis and its relevance for breast cancer. Cell adhesion & migration, 12(4), 305-314.

Richter, H., Satz, A. L., Bedoucha, M., Buettelmann, B., Petersen, A. C., Harmeier, A., ... & Prunotto, M. (2018). DNA-encoded library-derived DDR1 inhibitor prevents fibrosis and renal function loss in a genetic mouse model of Alport syndrome. ACS chemical biology, 14(1), 37-49.

Takai, K., Drain, A. P., Lawson, D. A., Littlepage, L. E., Karpuj, M., Kessenbrock, K., ... & Werb, Z. (2018). Discoidin domain receptor 1 (DDR1) ablation promotes tissue fibrosis and hypoxia to induce aggressive basal-like breast cancers. Genes & development, 32(3-4), 244-257.

Deng, Y., Zhao, F., Hui, L., Li, X., Zhang, D., Lin, W., ... & Ning, Y. (2017). Suppressing miR-199a-3p by promoter methylation contributes to tumor aggressiveness and cisplatin resistance of ovarian cancer through promoting DDR1 expression. Journal of ovarian research, 10(1), 1-11.

Juskaite, V., Corcoran, D. S., & Leitinger, B. (2017). Collagen induces activation of DDR1 through lateral dimer association and phosphorylation between dimers. Elife, 6, e25716.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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