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Mouse Anti-DFFB Recombinant Antibody (4H79) (CBMAB-D0753-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
4H79
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 310-338 at the C-terminus of human CAD.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DNA Fragmentation Factor Subunit Beta
Introduction
DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
Entrez Gene ID
1677
UniProt ID
O76075
Alternative Names
DNA Fragmentation Factor Subunit Beta; Caspase-Activated DNase; DNA Fragmentation Factor, 40kDa, Beta Polypeptide (Caspase-Activated DNase); DNA Fragmentation Factor 40 KDa Subunit; Caspase-Activated Deoxyribonuclease; Caspase-Activated Nuclease; DFF-40;
Function
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Biological Process
Apoptotic chromosome condensation Source: UniProtKB
Apoptotic DNA fragmentation Source: CAFA
DNA catabolic process Source: CAFA
Cellular Location
Nucleus; Cytoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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