Mouse Anti-DGKB Recombinant Antibody (CBYCD-251) (CBMAB-D0783-YC)

Name: Mouse Anti-DGKB Recombinant Antibody (CBYCD-251)
SKU: CBMAB-D0783-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBYCD-251

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 27-138 mapping near the N-terminus of human DGK-β.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Diacylglycerol Kinase Beta

Introduction

DGKB is a diacylglycerol kinase, beta isotype. Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD).

Entrez Gene ID

1607

UniProt ID

Q9Y6T7

Alternative Names

Diacylglycerol Kinase Beta; Diacylglycerol Kinase, Beta 90kDa; 90 KDa Diacylglycerol Kinase; Diglyceride Kinase Beta; DAG Kinase Beta; EC 2.7.1.107; DGK-BETA;

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:11719522).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Has a higher activity with long-chain diacylglycerols like 1,2-di-(9Z-octadecenoyl)-sn-glycerol compared to 1,2-didecanoyl-sn-glycerol (By similarity).

Specifically expressed in brain, it regulates neuron-specific morphological changes including neurite branching and neurite spine formation (By similarity).

Isoform 2:
Does not associate with membranes but has a diacylglycerol kinase activity.

Biological Process

Diacylglycerol metabolic process Source: UniProtKB
Glycerolipid metabolic process Source: BHF-UCL
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: BHF-UCL
Modulation of chemical synaptic transmission Source: Ensembl
Phosphatidic acid biosynthetic process Source: UniProtKB
Platelet activation Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
Regulation of postsynapse organization Source: Ensembl
Response to bacterium Source: Ensembl

Cellular Location

Postsynaptic cell membrane; Cell membrane; Cytoplasm. Translocation to the plasma membrane is induced by phorbol esters.
Isoform 2: Cytoplasm

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-DGKB Recombinant Antibody (1F9) (CAT#: V2LY-0425-LY243)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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