Mouse Anti-DGKG Recombinant Antibody (CBYCD-254) (V2LY-0425-LY248)

Name: Mouse Anti-DGKG Recombinant Antibody (CBYCD-254)
SKU: V2LY-0425-LY248
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-254

Application

Immunogen

E. coli-derived recombinant human DGK-gamma (Gly2-Ser126).

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	0.5 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

Trehalose & PBS

Preservative

None

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Diacylglycerol Kinase Gamma

Entrez Gene ID

Human	1608
Mouse	110197
Rat	25666

UniProt ID

Human	P49619
Mouse	Q91WG7
Rat	P49620

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:8034597).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (By similarity).

Has no apparent specificity with regard to the acyl compositions of diacylglycerol (PubMed:8034597).

Specifically expressed in the cerebellum where it controls the level of diacylglycerol which in turn regulates the activity of protein kinase C gamma. Through protein kinase C gamma, indirectly regulates the dendritic development of Purkinje cells, cerebellar long term depression and ultimately cerebellar motor coordination (By similarity).

Biological Process

Diacylglycerol metabolic process Source: UniProtKB
Glycerolipid metabolic process Source: BHF-UCL
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: UniProtKB
Negative regulation of protein kinase C signaling Source: UniProtKB
Neuron development Source: Ensembl
Phosphatidic acid biosynthetic process Source: UniProtKB
Platelet activation Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
Regulation of dendrite development Source: Ensembl

Cellular Location

Cytosol; Cytoskeleton; Membrane

Hattori, Y., Yamasaki, T., Ohashi, T., Miyanohana, Y., Kusumoto, T., Maeda, R., ... & Koike, T. (2021). Design, Synthesis, and Evaluation of 11C-Labeled 3-Acetyl-Indole Derivatives as a Novel Positron Emission Tomography Imaging Agent for Diacylglycerol Kinase Gamma (DGKγ) in Brain. Journal of Medicinal Chemistry, 64(16), 11990-12002.

Trinh, I., Gluscencova, O. B., & Boulianne, G. L. (2019). An in vivo screen for neuronal genes involved in obesity identifies Diacylglycerol kinase as a regulator of insulin secretion. Molecular metabolism, 19, 13-23.

Kai, M., Yamamoto, E., Sato, A., Yamano, H. O., Niinuma, T., Kitajima, H., ... & Suzuki, H. (2017). Epigenetic silencing of diacylglycerol kinase gamma in colorectal cancer. Molecular Carcinogenesis, 56(7), 1743-1752.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-DGKG Recombinant Antibody (CBYCD-254) (CAT#: CBMAB-D0789-YC)

Related Products

Mouse Anti-DGKG Recombinant Antibody (CBYCD-254) (CAT#: CBMAB-D0789-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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