Mouse Anti-DGKK Recombinant Antibody (4G12) (CBMAB-D0793-YC)

Name: Mouse Anti-DGKK Recombinant Antibody (4G12)
SKU: CBMAB-D0793-YC
Rating: 5 (1 reviews)

See all DGKK antibodies

Online Inquiry

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

4G12

Antibody Isotype

IgG2a, κ

Application

WB, IP, ELISA

Basic Information

Immunogen

Amino acids 1171-1271 representing partial length human DGK-κ.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Diacylglycerol Kinase Kappa

Introduction

DGKK is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias.

Entrez Gene ID

139189

UniProt ID

Q5KSL6

Alternative Names

Diacylglycerol Kinase Kappa; 142 KDa Diacylglycerol Kinase; Diglyceride Kinase Kappa; DAG Kinase Kappa; EC 2.7.1.107; DGK-Kappa;

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:16210324, PubMed:23949095).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable).

Biological Process

Diacylglycerol metabolic process Source: HGNC-UCL
Intracellular signal transduction Source: HGNC-UCL
Lipid phosphorylation Source: GO_Central
Platelet activation Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
Response to oxidative stress Source: HGNC-UCL

Cellular Location

Cell membrane

PTM

Phosphorylated at Tyr-78 by some member of the SRC family in response to H2O2.

More Infomation

References

Habbas, K., Cakil, O., Zámbó, B., Tabet, R., Riet, F., Dembele, D., ... & Moine, H. (2022). AAV‐delivered diacylglycerol kinase DGKk achieves long‐term rescue of fragile X syndrome mouse model. EMBO Molecular Medicine, e14649.

Hozyasz, K. K., Mostowska, A., Kowal, A., Mydlak, D., & Tsibulski, A. (2018). Further evidence of the association of the diacylglycerol kinase kappa (DGKK) gene with hypospadias. Urology Journal, 15(5), 272-276.

Xie, H., Lin, X. L., Zhang, S., Yu, L., Li, X. X., Huang, Y. C., ... & Chen, F. (2018). Association between diacylglycerol kinase kappa variants and hypospadias susceptibility in a Han Chinese population. Asian Journal of Andrology, 20(1), 85.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-DGKK Recombinant Antibody (4G12)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: