Mouse Anti-DHFR2 Recombinant Antibody (1E2) (V2LY-0425-LY273)

Name: Mouse Anti-DHFR2 Recombinant Antibody (1E2)
SKU: V2LY-0425-LY273
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Dog, Monkey

Clone

1E2

Antibody Isotype

IgG2b

Application

WB, IF

Basic Information

Immunogen

Human recombinant protein fragment corresponding to amino acids 4-185 of human DHFRL1 produced in E.coli

Host Species

Mouse

Specificity

Human, Mouse, Dog, Monkey

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IF(ICC)	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & PBS

Preservative

Sodium Azide

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Dihydrofolate Reductase 2

Entrez Gene ID

Human	200895
Mouse	111208

UniProt ID

Human

Q86XF0

Function

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR.

Biological Process

Dihydrofolate metabolic process Source: GO_Central
Folic acid metabolic process Source: GO_Central
Glycine biosynthetic process Source: InterPro
One-carbon metabolic process Source: UniProtKB-KW
Tetrahydrofolate biosynthetic process Source: GO_Central
Tetrahydrofolate metabolic process Source: UniProtKB
Thymidine biosynthetic process Source: UniProtKB

Cellular Location

Mitochondrion; Mitochondrion matrix; Mitochondrion inner membrane

More Infomation

References

Pangilinan, F., Finlay, E. K., Molloy, A. M., Abaan, H. O., Shane, B., Mills, J. L., ... & Parle-McDermott, A. (2021). A dihydrofolate reductase 2 (DHFR2) variant is associated with risk of neural tube defects in an Irish cohort but not in a United Kingdom cohort. American journal of medical genetics. Part A, 185(4), 1307-1311.

Kabir, E. R., Siam, M. K. S., Mustafa, N., & Kabir, S. M. (2018, December). Molecular docking reveals pitavastatin and related molecules antagonize 1DHF and its pseudogene DHFR2 in cancer treatment. In Proceedings of the 9th International Conference on Computational Systems-Biology and Bioinformatics (pp. 1-9).

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Mouse Anti-DHFR2 Recombinant Antibody (1E2)

Related Products

Mouse Anti-DHFR2 Recombinant Antibody (1E2)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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