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Mouse Anti-DHX38 Recombinant Antibody (3B9) (V2LY-0425-LY292)

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Published Data
Mouse Anti-DHX38 Recombinant Antibody (3B9) (V2LY-0425-LY292-1) in WB
Mouse Anti-DHX38 Recombinant Antibody (3B9) (V2LY-0425-LY292-1) in WB
DHX38 associates with multiple spliceosomal components.
DHX38WT-FLAG and DHX38G332D-FLAG were transiently expressed in HEK293 cells, immunoprecipitated and copurification of selected spliceosomal proteins monitored. Immunoprecipitation was performed in normal NET2 buffer containing 150 mM NaCl (A) or in stringent NET2 buffer containing 300 mM NaCl (B). ...View More
Obuća, M., Cvačková, Z., Kubovčiak, J., Kolář, M., & Staněk, D. (2022). Retinitis pigmentosa-linked mutation in DHX38 modulates its splicing activity. Plos one, 17(4), e0265742.
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Basic Information

Host Animal
Mouse
Clone
3B9
Application
WB, IP, IF, FC
Immunogen
N-terminus of human DDX38.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
DEAH (Asp-Glu-Ala-His) box polypeptide 38
Entrez Gene ID
Human9785
Mouse64340
Rat292007
UniProt ID
HumanQ92620
MouseQ80X98
RatD4A321
Function
Probable ATP-binding RNA helicase (Probable). Involved in pre-mRNA splicing as component of the spliceosome (PubMed:29301961, PubMed:9524131).
Biological Process
mRNA splicing, via spliceosome Source: UniProtKB
Cellular Location
Nucleus
Involvement in disease
Retinitis pigmentosa 84 (RP84):
A form of retinitis pigmentosa, a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP84 is an autosomal recessive, early onset form characterized by night blindness by age 4 and complete blindness by age 8. Funduscopy shows severely attenuated retinal vessels, severe macular atrophy, and prominent and deep macular colobomas lacking neuroretinal tissue.

Obuća, M., Cvačková, Z., Kubovčiak, J., Kolář, M., & Staněk, D. (2022). Retinitis pigmentosa-linked mutation in DHX38 modulates its splicing activity. Plos one, 17(4), e0265742.

Cona, B., Hayashi, T., Yamada, A., Shimizu, N., Yokota, N., Nakato, R., ... & Akiyama, T. (2022). The splicing factor DHX38/PRP16 is required for ovarian clear cell carcinoma tumorigenesis, as revealed by a CRISPR‐Cas9 screen. FEBS Open bio, 12(3), 582-593.

Latif, Z., Chakchouk, I., Schrauwen, I., Lee, K., Santos-Cortez, R. L. P., Abbe, I., ... & Leal, S. M. (2018). Confirmation of the role of DHX38 in the etiology of early-onset retinitis pigmentosa. Investigative Ophthalmology & Visual Science, 59(11), 4552-4557.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-DHX38 Recombinant Antibody (3B9) (CAT#: CBMAB-D0855-YC)

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Mouse Anti-DHX38 Recombinant Antibody (3B9) (CAT#: CBMAB-D0855-YC)

Mouse Anti-DHX38 Recombinant Antibody (8C117) (CAT#: V2LY-0425-LY293)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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