Mouse Anti-DHX58 Recombinant Antibody (CBT3343) (V2LY-0625-LY493)

Name: Mouse Anti-DHX58 Recombinant Antibody (CBT3343)
SKU: V2LY-0625-LY493
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-DHX58 Recombinant Antibody (CBT3343) (V2LY-0625-LY493) in ELISA

Mouse Anti-DHX58 Recombinant Antibody (CBT3343) (V2LY-0625-LY493) in WB

Mouse Anti-DHX58 Recombinant Antibody (CBT3343) (V2LY-0625-LY493) in FC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3343

Application

Immunogen

Purified recombinant fragment of human DHX58 (AA: 479-678) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DExH-Box Helicase 58

Entrez Gene ID

79132

UniProt ID

Q5TEU4

Function

Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses and coronavirus SARS-CoV-2, and also to the bacterial pathogen Listeria monocytogenes (PubMed:31256877).

Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.

Biological Process

Defense response to virus Source: UniProtKB-KW
Innate immune response Source: GO_Central
Negative regulation of innate immune response Source: UniProtKB
Negative regulation of MDA-5 signaling pathway Source: UniProtKB
Negative regulation of RIG-I signaling pathway Source: UniProtKB
Negative regulation of type I interferon production Source: UniProtKB
Positive regulation of interferon-beta production Source: GO_Central
Positive regulation of MDA-5 signaling pathway Source: UniProtKB
Positive regulation of RIG-I signaling pathway Source: UniProtKB
Positive regulation of type I interferon production Source: UniProtKB
Regulation of innate immune response Source: UniProtKB
Response to bacterium Source: Ensembl
Response to virus Source: UniProtKB

Cellular Location

Cytoplasm

Xu, J., Cai, Y., Ma, Z., Jiang, B., Liu, W., Cheng, J., ... & Li, Y. (2021). The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NFκB transcripts to dampen antiviral innate immunity. PLoS pathogens, 17(4), e1009530.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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