Mouse Anti-DMTF1 Recombinant Antibody (5C6) (V2LY-0425-LY591)

Name: Mouse Anti-DMTF1 Recombinant Antibody (5C6)
SKU: V2LY-0425-LY591
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse

Clone

5C6

Antibody Isotype

IgG2a, κ

Application

ELISA, IF, IHC-P, WB

Basic Information

Immunogen

DMTF1 (aa661-760) partial recombinant protein.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF(ICC)	10 µg/ml
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

DMTF1

Entrez Gene ID

9988

UniProt ID

Q9Y222

Function

Transcriptional activator which activates the CDKN2A/ARF locus in response to Ras-Raf signaling, thereby promoting p53/TP53-dependent growth arrest (By similarity).

Binds to the consensus sequence 5'-CCCG[GT]ATGT-3' (By similarity).

Isoform 1 may cooperate with MYB to activate transcription of the ANPEP gene. Isoform 2 may antagonize transcriptional activation by isoform 1.

Biological Process

Cell cycle Source: UniProtKB-KW
Regulation of transcription, DNA-templated Source: GO_Central
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

PTM

Phosphorylated by the cyclin-D2/CDK4, cyclin-D3/CDK4 and cyclin-D2/CDK6 complexes and to a lesser extent by the cyclin-D1/CDK4 complex.

More Infomation

References

Zhai, X., Wu, Y., Zhang, D., Li, H., Chong, T., & Zhao, J. (2021). MiR-6838-5p facilitates the proliferation and invasion of renal cell carcinoma cells through inhibiting the DMTF1/ARF-p53 axis. Journal of Bioenergetics and Biomembranes, 53(2), 191-202.

Li, J., Li, G., Qi, Y., Lu, Y., Wang, H., Shi, K., ... & Sui, G. (2021). SRSF5 regulates alternative splicing of DMTF1 pre-mRNA through modulating SF1 binding. RNA biology, 18(sup1), 318-336.

Fry, E. A., & Inoue, K. (2019). c-MYB and DMTF1 in cancer. Cancer investigation, 37(1), 46-65.

Yang, X., Lou, Y., Wang, M., Liu, C., Liu, Y., & Huang, W. (2019). miR‑675 promotes colorectal cancer cell growth dependent on tumor suppressor DMTF1. Molecular Medicine Reports, 19(3), 1481-1490.

Niklaus, N. J., Humbert, M., & Tschan, M. P. (2018). Cisplatin sensitivity in breast cancer cells is associated with particular DMTF1 splice variant expression. Biochemical and biophysical research communications, 503(4), 2800-2806.

Tian, N., Li, J., Shi, J., & Sui, G. (2017). From general aberrant alternative splicing in cancers and its therapeutic application to the discovery of an oncogenic DMTF1 isoform. International Journal of Molecular Sciences, 18(3), 191.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Mouse Anti-DMTF1 Recombinant Antibody (5C6)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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