Mouse Anti-DNAJC1 Recombinant Antibody (D1303) (V2LY-0425-LY644)

Name: Mouse Anti-DNAJC1 Recombinant Antibody (D1303)
SKU: V2LY-0425-LY644
Rating: 5 (1 reviews)

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Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat

Clone

D1303

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Amino acids 255-554 mapping at the C-terminus of human DnaJC1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

DnaJ Heat Shock Protein Family (Hsp40) Member C1

Entrez Gene ID

64215

UniProt ID

Q96KC8

Function

May modulate protein synthesis.

Biological Process

Negative regulation of proteolysis Source: UniProtKB
Protein folding Source: Ensembl
Regulation of protein secretion Source: UniProtKB
Regulation of translation Source: Ensembl

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane; Nucleus membrane

Topology

Lumenal: 48-153
Helical: 154-174
Cytoplasmic: 175-554

More Infomation

References

Wang, L. (2022). DNAJC1 inhibits ferroptosis by reducing ubiquitination of GPx4 in glioma cells. Brain Tumor Research and Treatment, 10(Suppl).

Meyer, N. J., Reilly, J. P., Anderson, B. J., Palakshappa, J. A., Shashaty, M. G., Ittner, C. A., ... & Christie, J. D. (2017). Genome Wide Analysis Of Sepsis-Associated Ards Implicates Dna J Heat Shock C1 (dnajc1). In C96. CRITICAL CARE: NEW DISCOVERIES IN ARDS (pp. A6750-A6750). American Thoracic Society.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-DNAJC1 Recombinant Antibody (D-10)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

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