Mouse Anti-DNAJC1 Recombinant Antibody (D1303) (V2LY-0425-LY644)

Name: Mouse Anti-DNAJC1 Recombinant Antibody (D1303)
SKU: V2LY-0425-LY644
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DNAJC1 Recombinant Antibody (D1303) (V2LY-0425-LY644-1) in WB

Knockdown of SINEUP RBPs. Numbers in the bottom row indicate knockdown efficiency compared with cells co-transfected with the SINEUP-GFP vector and negative control siRNA. ...View More

Toki, N., Takahashi, H., Sharma, H., Valentine, M. N., Rahman, F. U. M., Zucchelli, S., ... & Carninci, P. (2020). SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assemblies. Nucleic acids research, 48(20), 11626-11644.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D1303

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 255-554 mapping at the C-terminus of human DnaJC1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DnaJ Heat Shock Protein Family (Hsp40) Member C1

Entrez Gene ID

64215

UniProt ID

Q96KC8

Function

May modulate protein synthesis.

Biological Process

Negative regulation of proteolysis Source: UniProtKB
Protein folding Source: Ensembl
Regulation of protein secretion Source: UniProtKB
Regulation of translation Source: Ensembl

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane; Nucleus membrane

Topology

Lumenal: 48-153
Helical: 154-174
Cytoplasmic: 175-554

Wang, L. (2022). DNAJC1 inhibits ferroptosis by reducing ubiquitination of GPx4 in glioma cells. Brain Tumor Research and Treatment, 10(Suppl).

Meyer, N. J., Reilly, J. P., Anderson, B. J., Palakshappa, J. A., Shashaty, M. G., Ittner, C. A., ... & Christie, J. D. (2017). Genome Wide Analysis Of Sepsis-Associated Ards Implicates Dna J Heat Shock C1 (dnajc1). In C96. CRITICAL CARE: NEW DISCOVERIES IN ARDS (pp. A6750-A6750). American Thoracic Society.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Mouse Anti-DNAJC1 Recombinant Antibody (D-10) (CAT#: CBMAB-D1303-YC)

Isotype control

For research use only. Not intended for any clinical use.

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