Mouse Anti-DNAJC27 Recombinant Antibody (3C1) (CBMAB-D1314-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DnaJ Heat Shock Protein Family (Hsp40) Member C27 (DNAJC27). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DNAJC27 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3C1

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Immunogen

DNAJC27 (AAH34049, 1 a.a. ~ 273 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DnaJ (Hsp40) homolog, subfamily C, member 27

Introduction

DNAJC27 (DnaJ Heat Shock Protein Family (Hsp40) Member C27) is a protein coding gene. It has proved that the protein has GTP binding and GTPase activity. And a paralog is RAB18.

Entrez Gene ID

51277

UniProt ID

Q9NZQ0

Alternative Names

DnaJ Heat Shock Protein Family (Hsp40) Member C27; DnaJ (Hsp40) Homolog, Subfamily C, Member 27; Rab And DnaJ Domain-Containing Protein; RabJS; RBJ; DnaJ Homolog Subfamily C Member 27; Rab And DnaJ Domain Containing; Ras-Associated Protein Rap1;

Function

GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.

Biological Process

Intracellular protein transport Source: GO_Central
Positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Regulation of MAPK export from nucleus Source: Ensembl

Cellular Location

Nucleus

References

Hammad, M. M., Abu-Farha, M., Hebbar, P., Cherian, P., Al Khairi, I., Melhem, M., ... & Abubaker, J. (2020). MC4R variant rs17782313 associates with increased levels of DNAJC27, ghrelin, and visfatin and correlates with obesity and hypertension in a Kuwaiti cohort. Frontiers in Endocrinology, 11, 437.

Cherian, P. T., Al-Khairi, I., Sriraman, D., Al-Enezi, A., Al-Sultan, D., AlOtaibi, M., ... & Abu-Farha, M. (2018). Increased circulation and adipose tissue levels of DNAJC27/RBJ in obesity and type 2-diabetes. Frontiers in endocrinology, 9, 423.

Al Khairi, I., Sriraman, D., Cherian, P., Tuomilehto, J., Abubaker, J., & Abu-Farha, M. (2017). DnaJ Heat Shock Protein Family (Hsp40) Member C27 (DNAJC27) is a Heat Shock 40 protein (HSP40) family member that is increased the in obese people.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Datasheet
SDS
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