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Mouse Anti-DNAJC3 Recombinant Antibody (4D3-F2) (CBMAB-D1315-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DnaJ Heat Shock Protein Family (Hsp40) Member C3 (DNAJC3). The antibody can be used for immunoassay techniques, such as IF.
See all DNAJC3 antibodies
Published Data
Fig. 1 Immunoblot analysis of differential detergent extraction of CHOK1 cells transiently transfected with empty vector or p58( + ) construct.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
4D3-F2
Antibody Isotype
IgG2a, κ
Application
IF

Basic Information

Immunogen
DNAJC3 (AAH33823, 1 a.a. ~ 235 a.a) full length recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DnaJ Heat Shock Protein Family (Hsp40) Member C3
Introduction
DNAJC3 is a protein with multiple tetratricopeptide repeat (TPR) motifs as well as the highly conserved J domain found in DNAJ chaperone family members. It is a member of the tetratricopeptide repeat family of proteins and acts as an inhibitor of the interferon-induced, dsRNA-activated protein kinase (PKR).
Entrez Gene ID
5611
UniProt ID
Q13217
Alternative Names
DnaJ Heat Shock Protein Family (Hsp40) Member C3; Interferon-Induced, Double-Stranded RNA-Activated Protein Kinase Inhibitor; Endoplasmic Reticulum DNA J Domain-Containing Protein 6; Protein Kinase Inhibitor Of 58 KDa; DnaJ (Hsp40) Homolog, Subfamily C, Member 3; Protein Kinase Inhibitor P58; ER-Resident Protein ERdj6; P58IPK;
Function
Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity).

Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
Biological Process
Cellular response to cold Source: UniProtKB
Defense response to virus Source: UniProtKB-KW
Negative regulation of apoptotic process Source: ParkinsonsUK-UCL
Negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation Source: ParkinsonsUK-UCL
Positive regulation of translation initiation in response to endoplasmic reticulum stress Source: UniProtKB
Protein folding in endoplasmic reticulum Source: GO_Central
Proteolysis involved in cellular protein catabolic process Source: Ensembl
Response to unfolded protein Source: UniProtKB-KW
Cellular Location
Endoplasmic reticulum
Involvement in disease
Ataxia, combined cerebellar and peripheral, with hearing loss and diabetes mellitus (ACPHD):
A disease characterized by juvenile-onset diabetes and neurodegeneration, resulting in ataxia, upper-motor-neuron damage, peripheral neuropathy, hearing loss, and cerebral atrophy.

Li, H., Bi, K., Feng, S., Wang, Y., & Zhu, C. (2022). MiR-140 targets lncRNA DNAJC3-AS1 to suppress cell proliferation in acute myeloid leukemia. Mediterranean Journal of Hematology and Infectious Diseases, 14(1).

Lytrivi, M., Senée, V., Salpea, P., Fantuzzi, F., Philippi, A., Abdulkarim, B., ... & Cnop, M. (2021). DNAJC3 deficiency induces β-cell mitochondrial apoptosis and causes syndromic young-onset diabetes. European journal of endocrinology, 184(3), 459-472.

Tang, Y., Tang, R., Tang, M., Huang, P., Liao, Z., Zhou, J., ... & Nie, S. (2021). LncRNA DNAJC3-AS1 regulates fatty acid synthase via the EGFR pathway to promote the progression of colorectal cancer. Frontiers in oncology, 10, 3272.

Na, X. Y., Hu, X. Q., Zhao, Y., Hu, C. H., & Shang, X. S. (2021). LncRNA DNAJC3-AS1 functions as oncogene in renal cell carcinoma via regulation of the miR-27a-3p/PRDM14 axis. Eur Rev Med Pharmacol Sci, 25(3), 1291-1301.

Jennings, M. J., Hathazi, D., Nguyen, C. D., Munro, B., Münchberg, U., Ahrends, R., ... & Roos, A. (2021). Intracellular Lipid Accumulation and Mitochondrial Dysfunction Accompanies Endoplasmic Reticulum Stress Caused by Loss of the Co-chaperone DNAJC3. Frontiers in Cell and Developmental Biology, 9.

Fu, C., Li, J., Li, P., & Cheng, D. (2021). LncRNA DNAJC3-AS1 Promotes Hepatocellular Carcinoma (HCC) progression via sponging premature miR-27b. Cancer Management and Research, 13, 8575.

Han, B., Ge, Y., Cui, J., & Liu, B. (2020). Down-regulation of lncRNA DNAJC3-AS1 inhibits colon cancer via regulating miR-214-3p/LIVIN axis. Bioengineered, 11(1), 524-535.

Ozon, Z. A., Alikasifoglu, A., Kandemir, N., Aydin, B., Gonc, E. N., Karaosmanoglu, B., ... & Alikasifoglu, M. (2020). Novel insights into diabetes mellitus due to DNAJC3‐defect: Evolution of neurological and endocrine phenotype in the pediatric age group. Pediatric diabetes, 21(7), 1176-1182.

Liang, R., Liu, Z., Chen, Z., Yang, Y., Li, Y., Cui, Z., ... & Li, Q. (2019). Long noncoding RNA DNAJC3‐AS1 promotes osteosarcoma progression via its sense‐cognate gene DNAJC3. Cancer medicine, 8(2), 761-772.

Zhang, X., Li, G., Yang, X., Wang, L., Wang, Y., Guo, X., ... & Xu, B. (2019). Identification of a DnaJC3 gene in Apis cerana cerana and its involvement in various stress responses. Pesticide biochemistry and physiology, 160, 171-180.

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For research use only. Not intended for any clinical use.

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