Mouse Anti-DNMT1 Recombinant Antibody (CBT4540) (V2LY-0625-LY66)

Name: Mouse Anti-DNMT1 Recombinant Antibody (CBT4540)
SKU: V2LY-0625-LY66
Rating: 5 (1 reviews)

See all DNMT1 antibodies

Compare Online Inquiry

Tested Data

Mouse Anti-DNMT1 Recombinant Antibody (CBT4540) (V2LY-0625-LY66) in WB

Mouse Anti-DNMT1 Recombinant Antibody (CBT4540) (V2LY-0625-LY66) in IHC

Mouse Anti-DNMT1 Recombinant Antibody (CBT4540) (V2LY-0625-LY66) in ELISA

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4540

Application

WB, IHC

Immunogen

Purified recombinant fragment of human DNMT1 (AA: 1448-1594) expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Monkey

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DNA Methyltransferase 1

Entrez Gene ID

1786

UniProt ID

P26358

Function

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306).

Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306).

Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306).

Promotes tumor growth (PubMed:24623306).

Biological Process

Cellular response to amino acid stimulus Source: Ensembl
Chromatin organization Source: UniProtKB-KW
DNA methylation Source: ProtInc
DNA methylation involved in embryo development Source: Ensembl
Gene silencing Source: Ensembl
Maintenance of DNA methylation Source: UniProtKB
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of histone H3-K9 methylation Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: ProtInc
Negative regulation of vascular associated smooth muscle cell apoptotic process Source: BHF-UCL
Negative regulation of vascular associated smooth muscle cell differentiation involved in phenotypic switching Source: BHF-UCL
Positive regulation of DNA methylation-dependent heterochromatin assembly Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of histone H3-K4 methylation Source: UniProtKB
Positive regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Ras protein signal transduction Source: UniProtKB

Cellular Location

Nucleus

Involvement in disease

Neuropathy, hereditary sensory, 1E (HSN1E):
A neurodegenerative disorder characterized by adult onset of progressive peripheral sensory loss associated with progressive hearing impairment and early-onset dementia.
Cerebellar ataxia, deafness, and narcolepsy, autosomal dominant (ADCADN):
An autosomal dominant neurologic disorder characterized by adult onset of progressive cerebellar ataxia, narcolepsy, cataplexy, sensorineural deafness, and dementia. More variable features include optic atrophy, sensory neuropathy, psychosis, and depression.

PTM

Sumoylated; sumoylation increases activity.
Acetylation on multiple lysines, mainly by KAT2B/PCAF, regulates cell cycle G2/M transition. Deacetylation of Lys-1349 and Lys-1415 by SIRT1 increases methyltransferase activity.
Phosphorylation of Ser-154 by CDKs is important for enzymatic activity and protein stability. Phosphorylation of Ser-143 by AKT1 prevents methylation by SETD7 therebye increasing DNMT1 stability.
Methylation at Lys-142 by SETD7 promotes DNMT1 proteasomal degradation.
Ubiquitinated by UHRF1; interaction with USP7 counteracts ubiquitination by UHRF1 by promoting deubiquitination and preventing degradation by the proteasome.

Bayer, C., Pitschelatow, G., Hannemann, N., Linde, J., Reichard, J., Pensold, D., & Zimmer-Bensch, G. (2020). DNA Methyltransferase 1 (DNMT1) Acts on neurodegeneration by modulating proteostasis-relevant intracellular processes. International journal of molecular sciences, 21(15), 5420.

Wang, L., Lee, J. Y., Gao, L., Yin, J., Duan, Y., Jimenez, L. A., ... & Zhong, W. (2019). A DNA aptamer for binding and inhibition of DNA methyltransferase 1. Nucleic acids research, 47(22), 11527-11537.

Chen, L., Chan, L. S., Lung, H. L., Yip, T. T. C., Ngan, R. K. C., Wong, J. W. C., ... & Mak, N. K. (2019). Crucifera sulforaphane (SFN) inhibits the growth of nasopharyngeal carcinoma through DNA methyltransferase 1 (DNMT1)/Wnt inhibitory factor 1 (WIF1) axis. Phytomedicine, 63, 153058.

Li, Y., Chen, F., Chu, J., Wu, C., Li, Y., Li, H., & Ma, H. (2019). miR-148-3p inhibits growth of glioblastoma targeting DNA methyltransferase-1 (DNMT1). Oncology Research, 27(8), 911.

Yarychkivska, O., Shahabuddin, Z., Comfort, N., Boulard, M., & Bestor, T. H. (2018). BAH domains and a histone-like motif in DNA methyltransferase 1 (DNMT1) regulate de novo and maintenance methylation in vivo. Journal of Biological Chemistry, 293(50), 19466-19475.

Jung, H. J., Byun, H. O., Jee, B. A., Min, S., Jeoun, U. W., Lee, Y. K., ... & Yoon, G. (2017). The ubiquitin-like with PHD and ring finger domains 1 (UHRF1)/DNA methyltransferase 1 (DNMT1) axis is a primary regulator of cell senescence. Journal of Biological Chemistry, 292(9), 3729-3739.

Saini, S. K., Mangalhara, K. C., Prakasam, G., & Bamezai, R. N. K. (2017). DNA Methyltransferase1 (DNMT1) Isoform3 methylates mitochondrial genome and modulates its biology. Scientific reports, 7(1), 1-10.

Krishna, S., Shukla, S., Lakra, A. D., Meeran, S. M., & Siddiqi, M. I. (2017). Identification of potent inhibitors of DNA methyltransferase 1 (DNMT1) through a pharmacophore-based virtual screening approach. Journal of Molecular Graphics and Modelling, 75, 174-188.

Cai, Y., Tsai, H. C., Yen, R. W. C., Zhang, Y. W., Kong, X., Wang, W., ... & Baylin, S. B. (2017). Critical threshold levels of DNA methyltransferase 1 are required to maintain DNA methylation across the genome in human cancer cells. Genome research, 27(4), 533-544.

Pensold, D., Symmank, J., Hahn, A., Lingner, T., Salinas-Riester, G., Downie, B. R., ... & Zimmer, G. (2017). The DNA methyltransferase 1 (DNMT1) controls the shape and dynamics of migrating POA-derived interneurons fated for the murine cerebral cortex. Cerebral Cortex, 27(12), 5696-5714.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-DNMT1 Recombinant Antibody (CBT4540)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-DNMT1 Recombinant Antibody (D1398) (CAT#: CBMAB-D1399-YC)

Mouse Anti-DNMT1 Recombinant Antibody (CBYCD-364) (CAT#: V2LY-0425-LY718)

Rabbit Anti-DNMT1 Recombinant Antibody (10E20) (CAT#: CBMAB-D1403-YC)

Mouse Anti-DNMT1 Recombinant Antibody (D1401) (CAT#: CBMAB-D1401-YC)

Mouse Anti-DNMT1 Recombinant Antibody (CBT4483) (CAT#: V2LY-0625-LY513)

Mouse Anti-DNMT1 Recombinant Antibody (10F697) (CAT#: CBMAB-D1393-YC)

Mouse Anti-DNMT1 Recombinant Antibody (CBT3607) (CAT#: V2LY-0625-LY514)

Rabbit Anti-DNMT1 Recombinant Antibody (D1405) (CAT#: CBMAB-D1405-YC)

Mouse Anti-DNMT1 Recombinant Antibody (D1397) (CAT#: CBMAB-D1397-YC)

Mouse Anti-DNMT1 Recombinant Antibody (D1400) (CAT#: CBMAB-D1400-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry