Mouse Anti-DOC2A Recombinant Antibody (3C11) (CBMAB-D1469-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Double C2 Domain Alpha (DOC2A). The antibody can be used for immunoassay techniques, such as ELISA.
See all DOC2A antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

3C11

Antibody Isotype

IgG2b, κ

Application

ELISA

Basic Information

Immunogen

The immunogen sequence: MRGRRGDRMT INIQEHMAIN VCPGPIRPIR QISDYFPRGP GPEGGGGSGG EAPAHLVPLA LAPPAALLGA TTPEDGAEVD SYDSDDATAL GTLEFDLLYD RASCTLHCSI LRAKGLKPMD FNGLADPYVK LHLLPGACKA NKLKTKTQRN TLNPVWNEDL TYSGITDDDI THKVLRIAVC DEDKLSHNEF IGEIRVPLRR LKPSQKKHFN ICLERQVPLA SPSSMSAALR GISCYLKELE QAEQGQGLLE ERGRILLSLS YSSRRRGLLV GILRCAHLAA MDVNGYSDPY VKTYLRPDVD KKSKHKTCVK KKTLNPEFNE EFFYEIELST LATKTLEVTV WDYDIGKSND FIGGVSLGPG ARGEARKHWS DCLQQPDAAL ERWHTLTSEL PPAAGALSSA

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 1-400

Target

Full Name

double C2-like domains, alpha

Introduction

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release.

Entrez Gene ID

8448

UniProt ID

Q14183

Alternative Names

Double C2 Domain Alpha; Double C2-Like Domains, Alpha; Doc2-Alpha; DOC2; Double C2-Like Domain-Containing Protein Alpha;

Function

Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca2+-dependent secretory lysosome exocytosis in mast cells.

Biological Process

Chemical synaptic transmission Source: ProtInc
Nervous system development Source: ProtInc
Regulation of calcium ion-dependent exocytosis Source: UniProtKB
Spontaneous neurotransmitter secretion Source: Ensembl
Synaptic vesicle exocytosis Source: Ensembl

Cellular Location

Lysosome; Synaptic vesicle membrane; Synaptosome

References

Sahid, M. N., Liu, S., Kiyoi, T., Maeyama, K., & Mogi, M. (2021). Inhibition of Histamine Release from RBL-2H3 Cells by Zoledronate Did Not Affect Rab27a/Doc2a Interaction. Biological and Pharmaceutical Bulletin, 44(12), 1902-1906.

Hu, X., Tang, J., Lan, X., & Mi, X. (2019). Increased expression of DOC2A in human and rat temporal lobe epilepsy. Epilepsy Research, 151, 78-84.

Sahid, M. N., Liu, S., Kiyoi, T., & Maeyama, K. (2018). Zoledronate and clodronate inhibited RBL-2H3 cells degranulation without disturbing Rab27a-Doc2a interaction. In Proceedings for Annual Meeting of The Japanese Pharmacological Society WCP2018 (The 18th World Congress of Basic and Clinical Pharmacology) (pp. PO3-4). Japanese Pharmacological Society.

McCammon, J. M., Blaker-Lee, A., Chen, X., & Sive, H. (2017). The 16p11. 2 homologs fam57ba and doc2a generate certain brain and body phenotypes. Human molecular genetics, 26(19), 3699-3712.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-DOC2A Recombinant Antibody (3C11)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: