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Mouse Anti-DOCK2 Recombinant Antibody (4E12) (V2LY-0425-LY769)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
4E12
Antibody Isotype
IgG2a
Application
IHC-P

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 1544-1830 of human DOCK2 produced in E.coli.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
IHC-P1:150

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & PBS
Preservative
Sodium Azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
dedicator of cytokinesis 2
Entrez Gene ID
1794
UniProt ID
Q92608
Function
Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.
Biological Process
Actin cytoskeleton organization Source: UniProtKB
Alpha-beta T cell proliferation Source: Ensembl
Chemotaxis Source: Ensembl
Establishment of T cell polarity Source: Ensembl
Immunological synapse formation Source: Ensembl
Macropinocytosis Source: UniProtKB
Membrane raft polarization Source: Ensembl
Myeloid dendritic cell activation involved in immune response Source: UniProtKB
Negative thymic T cell selection Source: Ensembl
Positive regulation of phagocytosis Source: UniProtKB
Positive thymic T cell selection Source: Ensembl
Regulation of small GTPase mediated signal transduction Source: Reactome
Small GTPase mediated signal transduction Source: InterPro
Cellular Location
Cytoskeleton; Endomembrane system. Colocalizes with F-actin.
Involvement in disease
Immunodeficiency 40 (IMD40):
A form of combined immunodeficiency characterized by lymphopenia, and defective T-cell, B-cell, and NK-cell responses. Patients suffer from severe invasive bacterial and viral infections in early childhood and may die without bone marrow transplantation.
More Infomation

Qian, G., Adeyanju, O., Roy, S., Sunil, C., Jeffers, A., Guo, X., ... & Tucker, T. A. (2022). DOCK2 promotes pleural fibrosis by modulating mesothelial to mesenchymal transition. American journal of respiratory cell and molecular biology, 66(2), 171-182.

Xu, X., Su, Y., Wu, K., Pan, F., & Wang, A. (2021). DOCK2 contributes to endotoxemia-induced acute lung injury in mice by activating proinflammatory macrophages. Biochemical Pharmacology, 184, 114399.

Chang, L., Yang, J., Jo, C. H., Boland, A., Zhang, Z., McLaughlin, S. H., ... & Barford, D. (2020). Structure of the DOCK2− ELMO1 complex provides insights into regulation of the auto-inhibited state. Nature communications, 11(1), 1-17.

Bjerre, M. T., Strand, S. H., Nørgaard, M., Kristensen, H., Rasmussen, A. K., Mortensen, M. M., ... & Sørensen, K. D. (2019). Aberrant DOCK2, GRASP, HIF3A and PKFP hypermethylation has potential as a prognostic biomarker for prostate cancer. International journal of molecular sciences, 20(5), 1173.

Moens, L., Gouwy, M., Bosch, B., Pastukhov, O., Nieto-Patlàn, A., Siler, U., ... & Meyts, I. (2019). Human DOCK2 deficiency: report of a novel mutation and evidence for neutrophil dysfunction. Journal of clinical immunology, 39(3), 298-308.

Hu, N., Pang, Y., Zhao, H., Si, C., Ding, H., Chen, L., ... & Fu, L. (2019). High expression of DOCK2 indicates good prognosis in acute myeloid leukemia. Journal of Cancer, 10(24), 6088.

Chen, Y., Meng, F., Wang, B., He, L., Liu, Y., & Liu, Z. (2018). Dock2 in the development of inflammation and cancer. European journal of immunology, 48(6), 915-922.

Alizadeh, Z., Mazinani, M., Shakerian, L., Nabavi, M., & Fazlollahi, M. R. (2018). DOCK2 deficiency in a patient with hyper IgM phenotype. Journal of Clinical Immunology, 38(1), 10-12.

Hasan, M. K., Yu, J., Widhopf, G. F., Rassenti, L. Z., Chen, L., Shen, Z., ... & Kipps, T. J. (2018). Wnt5a induces ROR1 to recruit DOCK2 to activate Rac1/2 in chronic lymphocytic leukemia. Blood, The Journal of the American Society of Hematology, 132(2), 170-178.

Sakurai, T., Uruno, T., Sugiura, Y., Tatsuguchi, T., Yamamura, K., Ushijima, M., ... & Fukui, Y. (2018). Cholesterol sulfate is a DOCK2 inhibitor that mediates tissue-specific immune evasion in the eye. Science signaling, 11(541), eaao4874.

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For research use only. Not intended for any clinical use.

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