Mouse Anti-DOCK2 Recombinant Antibody (D1475) (V2LY-0425-LY767)

Name: Mouse Anti-DOCK2 Recombinant Antibody (D1475)
SKU: V2LY-0425-LY767
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DOCK2 Recombinant Antibody (D1475) (V2LY-0425-LY767-1) in Immunoblot

a Immunoblot analysis of lysates prepared from freshly-isolated primary CLL cells (representative of 3 patients) transfected 72 h previously with control siRNA or siRNA targeting DOCK2, then treated without (-) or with (+) Wnt5a; expression of DOCK2, total ERK1/2, AKT, and activated pERK1/2, pAKT was measured, as indicated on the left. The numbers between two lanes are ratios of band IOD of pERK1/2 or pAKT versus total ERK1/2 or AKT respectively, and normalized with respect to that of untreated cells. b Immunoblot analysis of lysates prepared from MEC1-ROR1 cells transfected 72 h previously with control siRNA or siRNA targeting DOCK2; expression of DOCK2, total ERK1/2, AKT, and activated pERK1/2, pAKT was measured, as indicated on the left. ...View More

Hasan, M. K., Ghia, E. M., Rassenti, L. Z., Widhopf, G. F., & Kipps, T. J. (2021). Wnt5a enhances proliferation of chronic lymphocytic leukemia and ERK1/2 phosphorylation via a ROR1/DOCK2-dependent mechanism. Leukemia, 35(6), 1621-1630.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D1475

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 796-894 mapping within an internal region of human DOCK2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

dedicator of cytokinesis 2

Entrez Gene ID

1794

UniProt ID

Q92608

Function

Involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. Activates RAC1 and RAC2, but not CDC42, by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. May also participate in IL2 transcriptional activation via the activation of RAC2.

Biological Process

Actin cytoskeleton organization Source: UniProtKB
Alpha-beta T cell proliferation Source: Ensembl
Chemotaxis Source: Ensembl
Establishment of T cell polarity Source: Ensembl
Immunological synapse formation Source: Ensembl
Macropinocytosis Source: UniProtKB
Membrane raft polarization Source: Ensembl
Myeloid dendritic cell activation involved in immune response Source: UniProtKB
Negative thymic T cell selection Source: Ensembl
Positive regulation of phagocytosis Source: UniProtKB
Positive thymic T cell selection Source: Ensembl
Regulation of small GTPase mediated signal transduction Source: Reactome
Small GTPase mediated signal transduction Source: InterPro

Cellular Location

Cytoskeleton; Endomembrane system. Colocalizes with F-actin.

Involvement in disease

Immunodeficiency 40 (IMD40):
A form of combined immunodeficiency characterized by lymphopenia, and defective T-cell, B-cell, and NK-cell responses. Patients suffer from severe invasive bacterial and viral infections in early childhood and may die without bone marrow transplantation.

Qian, G., Adeyanju, O., Roy, S., Sunil, C., Jeffers, A., Guo, X., ... & Tucker, T. A. (2022). DOCK2 promotes pleural fibrosis by modulating mesothelial to mesenchymal transition. American journal of respiratory cell and molecular biology, 66(2), 171-182.

Xu, X., Su, Y., Wu, K., Pan, F., & Wang, A. (2021). DOCK2 contributes to endotoxemia-induced acute lung injury in mice by activating proinflammatory macrophages. Biochemical Pharmacology, 184, 114399.

Chang, L., Yang, J., Jo, C. H., Boland, A., Zhang, Z., McLaughlin, S. H., ... & Barford, D. (2020). Structure of the DOCK2− ELMO1 complex provides insights into regulation of the auto-inhibited state. Nature communications, 11(1), 1-17.

Bjerre, M. T., Strand, S. H., Nørgaard, M., Kristensen, H., Rasmussen, A. K., Mortensen, M. M., ... & Sørensen, K. D. (2019). Aberrant DOCK2, GRASP, HIF3A and PKFP hypermethylation has potential as a prognostic biomarker for prostate cancer. International journal of molecular sciences, 20(5), 1173.

Moens, L., Gouwy, M., Bosch, B., Pastukhov, O., Nieto-Patlàn, A., Siler, U., ... & Meyts, I. (2019). Human DOCK2 deficiency: report of a novel mutation and evidence for neutrophil dysfunction. Journal of clinical immunology, 39(3), 298-308.

Hu, N., Pang, Y., Zhao, H., Si, C., Ding, H., Chen, L., ... & Fu, L. (2019). High expression of DOCK2 indicates good prognosis in acute myeloid leukemia. Journal of Cancer, 10(24), 6088.

Chen, Y., Meng, F., Wang, B., He, L., Liu, Y., & Liu, Z. (2018). Dock2 in the development of inflammation and cancer. European journal of immunology, 48(6), 915-922.

Alizadeh, Z., Mazinani, M., Shakerian, L., Nabavi, M., & Fazlollahi, M. R. (2018). DOCK2 deficiency in a patient with hyper IgM phenotype. Journal of Clinical Immunology, 38(1), 10-12.

Hasan, M. K., Yu, J., Widhopf, G. F., Rassenti, L. Z., Chen, L., Shen, Z., ... & Kipps, T. J. (2018). Wnt5a induces ROR1 to recruit DOCK2 to activate Rac1/2 in chronic lymphocytic leukemia. Blood, The Journal of the American Society of Hematology, 132(2), 170-178.

Sakurai, T., Uruno, T., Sugiura, Y., Tatsuguchi, T., Yamamura, K., Ushijima, M., ... & Fukui, Y. (2018). Cholesterol sulfate is a DOCK2 inhibitor that mediates tissue-specific immune evasion in the eye. Science signaling, 11(541), eaao4874.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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