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Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY)

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Published Data
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-1) in Immunoblot
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-1) in Immunoblot
Immunoblot of classical cadherins (Pan-Cad), desmosomal cadherins (DSG1, DSG2, DSG3), and plakoglobin (PG) in lysates from NHEKs treated with dabrafenib (Dab) or Vemurafenib (Vem) ± Trametinib (Tram) for 24 hours; β-actin is a loading control. ...View More
Simpson, C. L., Tiwaa, A., Zaver, S. A., Johnson, C. J., Chu, E. Y., Harms, P. W., & Gudjonsson, J. E. (2024). ERK hyperactivation in epidermal keratinocytes impairs intercellular adhesion and drives Grover disease pathology. JCI insight, 9(21), e182983.
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-2) in IF
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-2) in IF
Immunostaining of DSG1 (green) and plakoglobin (magenta) in tissue cross sections from patient biopsies; images shown are from 2 control donors and 2 patients with drug-induced GD and are representative of n = 5 patients in each group; Scale bar: 50 μm. ...View More
Simpson, C. L., Tiwaa, A., Zaver, S. A., Johnson, C. J., Chu, E. Y., Harms, P. W., & Gudjonsson, J. E. (2024). ERK hyperactivation in epidermal keratinocytes impairs intercellular adhesion and drives Grover disease pathology. JCI insight, 9(21), e182983.
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-3) in IHC
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-3) in IHC
The expression of desmoglein 1 (DSG1), desmoglein 3 (DSG3), and E-cadherin (E-cad) in multiple pemphigus vulgaris cases: A) positive DSG3 expression; B) negative DSG3 expression; C) strong positive DSG1 expression; D) weak positive DSG1 expression; E) negative DSG1 expression; F) strong E-cad expression; G) moderate E-cad expression; H) mild E-cad expression; and I) negative E-cad expression. ...View More
Alshami, M. L., Aswad, F., & Abdullah, B. (2022). Desmogleins 1, 3, and E-cadherin immunohistochemical expression within mucocutaneous pemphigus vulgaris. Pan African Medical Journal, 42(1).
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-4) in WB
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-4) in WB
Western blot analysis of the fractions was performed to detect Dsg3 and Dsg1. Analysis of GAPDH level was included to ensure equal loading and the purity of the detergent insoluble fraction. Equal percentage of each fraction was loaded. ...View More
Zakrzewicz, A., Würth, C., Beckert, B., Feldhoff, S., Vanderheyden, K., Foss, S., ... & Tikkanen, R. (2022). Stabilization of keratinocyte monolayer integrity in the presence of anti-desmoglein-3 antibodies through FcRn blockade with efgartigimod: novel treatment paradigm for pemphigus?. Cells, 11(6), 942.
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-5) in IF
Mouse Anti-DSG1 Recombinant Antibody (CBFYA-0124) (CBMAB-X0181-FY-5) in IF
Effect of calcium concentration on the localization of desmogleins in human telomerase reverse transcriptase (hTert) cells. The cells were grown on coverslips in Keratinocyte growth medium (KGM) with 0.05 mM calcium for at least four days, and then shifted to 2 mM calcium for 24 h. After methanol (MeOH) fixation, the cells were stained with anti-desmoglein (Dsg) antibodies and fluorochrome coupled secondary antibodies (anti-mouse Alexa488, green). The coverslips were mounted in a mounting medium with 4',6-diamidino-2-phenylindole (DAPI, blue) to visualize nuclei. n = 4 independent experiments, scale bar 20 µm. ...View More
Beckert, B., Panico, F., Pollmann, R., Eming, R., Banning, A., & Tikkanen, R. (2019). Immortalized human hTert/KER-CT keratinocytes a model system for research on desmosomal adhesion and pathogenesis of pemphigus vulgaris. International journal of molecular sciences, 20(13), 3113.
Datasheet
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Basic Information

Host Animal
Mouse
Clone
CBFYA-0124
Application
WB, IP, IF, ELISA, IHC-P
Immunogen
Amino acids 760-1049 mapping near the C-terminus of human dsg1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Desmoglein 1
Introduction
This gene encodes a member of the desmoglein protein subfamily. Desmogleins, along with desmocollins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmoglein family members on chromosome 18. The encoded protein has been identified as a target of auto-antibodies in the autoimmune skin blistering disease pemphigus foliaceus. Disruption of this gene has also been associated with the skin diseases palmoplantar keratoderma and erythroderma.
Entrez Gene ID
Human1828
Mouse13510
Rat291755
UniProt ID
HumanQ02413
MouseQ61495
RatD4A4T7
Alternative Names
Desmoglein 1; Pemphigus Foliaceus Antigen; Desmosomal Glycoprotein 1; Cadherin Family Member 4; CDHF4; DG1; Desmoglein-1
Research Area
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Biological Process
Calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Cell-cell adhesion Source: GO_Central
Cell-cell junction assembly Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: InterPro
Protein stabilization Source: BHF-UCL
Cellular Location
Cell membrane; Desmosome
Involvement in disease
Palmoplantar keratoderma 1, striate, focal, or diffuse (PPKS1):
A dermatological disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger.
Erythroderma, congenital, with palmoplantar keratoderma, hypotrichosis, and hyper IgE (EPKHE):
A syndrome characterized by severe dermatitis, multiple allergies and metabolic wasting. Clinical features include erythroderma, yellowish papules and plaques arranged at the periphery of the palms, along the fingers and over weight-bearing areas of the feet, skin erosions and scaling, and hypotrichosis. Additionally, patients manifest severe food allergies, elevated immunoglobulin E (IgE) levels and recurrent infections with marked metabolic wasting.
Topology
Extracellular: 50-548
Helical: 549-569
Cytoplasmic: 570-1049

Zimmer, S. E., Takeichi, T., Conway, D. E., Kubo, A., Suga, Y., Akiyama, M., & Kowalczyk, A. P. (2022). Differential Pathomechanisms of Desmoglein 1 Transmembrane Domain Mutations in Skin Disease. Journal of Investigative Dermatology, 142(2), 323-332.

Delavarian, Z., Layegh, P., Pakfetrat, A., Zarghi, N., Khorashadizadeh, M., & Ghazi, A. (2020). Evaluation of desmoglein 1 and 3 autoantibodies in pemphigus vulgaris: Correlation with disease severity. Journal of Clinical and Experimental Dentistry, 12(5), e440.

Arnette, C. R., Roth‐Carter, Q. R., Koetsier, J. L., Broussard, J. A., Burks, H. E., Cheng, K., ... & Green, K. J. (2020). Keratinocyte cadherin desmoglein 1 controls melanocyte behavior through paracrine signaling. Pigment cell & melanoma research, 33(2), 305-317.

Cohen-Barak, E., Godsel, L. M., Koetsier, J. L., Hegazy, M., Kushnir-Grinbaum, D., Hammad, H., ... & Green, K. J. (2020). The role of desmoglein 1 in gap junction turnover revealed through the study of SAM syndrome. Journal of Investigative Dermatology, 140(3), 556-567.

Valenzuela-Iglesias, A., Burks, H. E., Arnette, C. R., Yalamanchili, A., Nekrasova, O., Godsel, L. M., & Green, K. J. (2019). Desmoglein 1 regulates invadopodia by suppressing EGFR/Erk signaling in an Erbin-dependent manner. Molecular Cancer Research, 17(5), 1195-1206.

Evangelista, F., Roth, A. J., Prisayanh, P., Temple, B. R., Li, N., Qian, Y., ... & Diaz, L. A. (2018). Pathogenic IgG4 autoantibodies from endemic pemphigus foliaceus recognize a desmoglein-1 conformational epitope. Journal of autoimmunity, 89, 171-185.

Polivka, L., Hadj-Rabia, S., Bal, E., Leclerc-Mercier, S., Madrange, M., Hamel, Y., ... & Smahi, A. (2018). Epithelial barrier dysfunction in desmoglein-1 deficiency. Journal of Allergy and Clinical Immunology, 142(2), 702-706.

Lovgren, M. L., McAleer, M. A., Irvine, A. D., Wilson, N. J., Tavadia, S., Schwartz, M. E., ... & Zamiri, M. (2017). Mutations in desmoglein 1 cause diverse inherited palmoplantar keratoderma phenotypes: implications for genetic screening. British Journal of Dermatology, 176(5), 1345-1350.

Yoshida, K., Ishii, K., Shimizu, A., Yokouchi, M., Amagai, M., Shiraishi, K., ... & Ishiko, A. (2017). Non-pathogenic pemphigus foliaceus (PF) IgG acts synergistically with a directly pathogenic PF IgG to increase blistering by p38MAPK-dependent desmoglein 1 clustering. Journal of dermatological science, 85(3), 197-207.

Totsuka, A., Omori-Miyake, M., Kawashima, M., Yagi, J., & Tsunemi, Y. (2017). Expression of keratin 1, keratin 10, desmoglein 1 and desmocollin 1 in the epidermis: possible downregulation by interleukin-4 and interleukin-13 in atopic dermatitis. European Journal of Dermatology, 27(3), 247-253.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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