Mouse Anti-DTD2 Recombinant Antibody (CBYCD-433) (CBMAB-D1832-YC)

Name: Mouse Anti-DTD2 Recombinant Antibody (CBYCD-433)
SKU: CBMAB-D1832-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-433

Application

ELISA

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

D-Tyrosyl-TRNA Deacylase 2 (Putative)

Introduction

DTD2 (D-Tyrosyl-TRNA Deacylase 2 (Putative)) is a protein coding gene. It has proved that the protein has hydrolase activity, acting on ester bonds and D-tyrosyl-tRNA(Tyr) deacylase activity. And a paralog is ENSG00000203546.

Entrez Gene ID

112487

UniProt ID

Q96FN9

Alternative Names

D-Tyrosyl-TRNA Deacylase 2 (Putative); Putative D-Tyrosyl-TRNA Deacylase 2; C14orf126; Probable D-Tyrosyl-TRNA(Tyr) Deacylase 2; Putative D-Tyrosyl-TRNA(Tyr) Deacylase 2; Chromosome 14 Open Reading Frame 126; EC 3.1.-.-;

Research Area

Deacylates mischarged D-aminoacyl-tRNAs (By similarity).

Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS (By similarity).

Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids (By similarity).

Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr) (By similarity).

By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS (PubMed:29410408).

Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site (PubMed:29410408).

Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation (By similarity).

Biological Process

Aminoacyl-tRNA metabolism involved in translational fidelity Source: UniProtKB
tRNA metabolic process Source: GO_Central

Cellular Location

Cytoplasm

Gogoi, J., Bhatnagar, A., Ann, K. J., Pottabathini, S., Singh, R., Mazeed, M., ... & Sankaranarayanan, R. (2022). Switching a conflicted bacterial DTD-tRNA code is essential for the emergence of mitochondria. Science advances, 8(2), eabj7307.

Mazeed, M., Singh, R., Kumar, P., Roy, A., Raman, B., Kruparani, S. P., & Sankaranarayanan, R. (2021). Recruitment of archaeal DTD is a key event toward the emergence of land plants. Science Advances, 7(6), eabe8890.

Kuncha, S. K., Mazeed, M., Singh, R., Kattula, B., Routh, S. B., & Sankaranarayanan, R. (2018). A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia. Nature communications, 9(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-DTD2 Recombinant Antibody (CBYCD-433) (CAT#: V2LY-0425-LY1025)

Related Products

Mouse Anti-DTD2 Recombinant Antibody (CBYCD-433) (CAT#: V2LY-0425-LY1025)

Isotype control

For research use only. Not intended for any clinical use.

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