Mouse Anti-DTD2 Recombinant Antibody (CBYCD-433) (V2LY-0425-LY1025)

Name: Mouse Anti-DTD2 Recombinant Antibody (CBYCD-433)
SKU: V2LY-0425-LY1025
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-433

Application

ELISA

Immunogen

Full-length recombinant C14orf126.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

D-Tyrosyl-TRNA Deacylase 2 (Putative)

Entrez Gene ID

112487

UniProt ID

Q96FN9

Research Area

Deacylates mischarged D-aminoacyl-tRNAs (By similarity).

Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS (By similarity).

Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids (By similarity).

Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr) (By similarity).

By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS (PubMed:29410408).

Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site (PubMed:29410408).

Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation (By similarity).

Biological Process

Aminoacyl-tRNA metabolism involved in translational fidelity Source: UniProtKB
tRNA metabolic process Source: GO_Central

Cellular Location

Cytoplasm

Gogoi, J., Bhatnagar, A., Ann, K. J., Pottabathini, S., Singh, R., Mazeed, M., ... & Sankaranarayanan, R. (2022). Switching a conflicted bacterial DTD-tRNA code is essential for the emergence of mitochondria. Science advances, 8(2), eabj7307.

Mazeed, M., Singh, R., Kumar, P., Roy, A., Raman, B., Kruparani, S. P., & Sankaranarayanan, R. (2021). Recruitment of archaeal DTD is a key event toward the emergence of land plants. Science Advances, 7(6), eabe8890.

Kuncha, S. K., Mazeed, M., Singh, R., Kattula, B., Routh, S. B., & Sankaranarayanan, R. (2018). A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia. Nature communications, 9(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-DTD2 Recombinant Antibody (CBYCD-433) (CAT#: CBMAB-D1832-YC)

Related Products

Mouse Anti-DTD2 Recombinant Antibody (CBYCD-433) (CAT#: CBMAB-D1832-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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