Mouse Anti-DYRK1A Recombinant Antibody (CBYCD-463) (V2LY-0425-LY1151)

Name: Mouse Anti-DYRK1A Recombinant Antibody (CBYCD-463)
SKU: V2LY-0425-LY1151
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Published Data

Mouse Anti-DYRK1A Recombinant Antibody (CBYCD-463) (V2LY-0425-LY1151-1) in WB

Differentiated C2C12 myotubes were treated with 0.1% DMSO or 1.3 µg/ml harmol for the indicated times, and whole protein lysates were analyzed by Western blot for he indicated proteins. Band quantification is shown to the right. ...View More

Costa-Machado, L. F., Garcia-Dominguez, E., McIntyre, R. L., Lopez-Aceituno, J. L., Ballesteros-Gonzalez, Á., Tapia-Gonzalez, A., ... & Fernandez-Marcos, P. J. (2023). Peripheral modulation of antidepressant targets MAO-B and GABAAR by harmol induces mitohormesis and delays aging in preclinical models. Nature Communications, 14(1), 2779.

Mouse Anti-DYRK1A Recombinant Antibody (CBYCD-463) (V2LY-0425-LY1151-2) in Immunoblot

Exponentially growing MDA-MB-231 cells were treated with Sunitinib (3 μM) for the indicated times. Whole-cell lysates were resolved in SDS-PAGE and immunoblotted for the indicated proteins. Representative blot of n=3 independent experiments. ...View More

Paul, D., Kales, S. C., Cornwell, J. A., Afifi, M. M., Rai, G., Zakharov, A., ... & Cappell, S. D. (2022). Revealing β-TrCP activity dynamics in live cells with a genetically encoded biosensor. Nature Communications, 13(1), 6364.

Mouse Anti-DYRK1A Recombinant Antibody (CBYCD-463) (V2LY-0425-LY1151-3) in Immunoblot

Immunoblot images of DYRK1A expression in MMK1 and HW1 cells transfected with scramble (si Scr) and DYRK1A (si DYR) targeting siRNA, cultured with GF or BMP4 (7 days). ...View More

Chen, B., McCuaig-Walton, D., Tan, S., Montgomery, A. P., Day, B. W., Kassiou, M., ... & Recasens, A. (2021). DYRK1A negatively regulates CDK5-SOX2 pathway and self-renewal of glioblastoma stem cells. International journal of molecular sciences, 22(8), 4011.

Mouse Anti-DYRK1A Recombinant Antibody (CBYCD-463) (V2LY-0425-LY1151-4) in WB

Representative WB showing the expression level of CDKL5, DYRK1A, total Tau and Tau phosphorylated on T212 in WT/Cdkl5-KO neurons or KO neurons treated with 1 μM EGCG or 0.1 μM harmine from DIV7-DIV10. GAPDH was used as internal standard. ...View More

Trovò, L., Fuchs, C., De Rosa, R., Barbiero, I., Tramarin, M., Ciani, E., ... & Kilstrup-Nielsen, C. (2020). The green tea polyphenol epigallocatechin-3-gallate (EGCG) restores CDKL5-dependent synaptic defects in vitro and in vivo. Neurobiology of Disease, 138, 104791.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYCD-463

Application

ELISA, WB, IP

Immunogen

Amino acids 674-763 of human Dyrk1A.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A

Entrez Gene ID

Human	1859
Mouse	13548
Rat	25255

UniProt ID

Human	Q13627
Mouse	Q61214
Rat	Q63470

Research Area

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity).

Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity).

Biological Process

Amyloid-beta formation Source: ARUK-UCL
Circadian rhythm Source: UniProtKB
Negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
Negative regulation of microtubule polymerization Source: ARUK-UCL
Negative regulation of mRNA splicing, via spliceosome Source: Ensembl
Nervous system development Source: ProtInc
Peptidyl-serine autophosphorylation Source: ARUK-UCL
Peptidyl-serine phosphorylation Source: ARUK-UCL
Peptidyl-threonine phosphorylation Source: BHF-UCL
Peptidyl-tyrosine autophosphorylation Source: ARUK-UCL
Peptidyl-tyrosine phosphorylation Source: MGI
Positive regulation of protein deacetylation Source: BHF-UCL
Positive regulation of RNA splicing Source: ARUK-UCL
Positive regulation of transcription, DNA-templated Source: GO_Central
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of alternative mRNA splicing, via spliceosome Source: Ensembl

Cellular Location

Nucleus; Nucleus speckle

Involvement in disease

Mental retardation, autosomal dominant 7 (MRD7):
A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.

PTM

Autophosphorylated on numerous tyrosine residues. Can also autophosphorylate on serine and threonine residues (in vitro).

Bessone, I. F., Navarro, J., Martinez, E., Karmirian, K., Holubiec, M., Alloatti, M., ... & Falzone, T. L. (2022). DYRK1A regulates the bidirectional axonal transport of APP in human-derived neurons. Journal of Neuroscience, 42(33), 6344-6358.

Weber, C., Sipos, M., Paczal, A., Balint, B., Kun, V., Foloppe, N., ... & Kotschy, A. (2021). Structure-guided discovery of potent and selective DYRK1A inhibitors. Journal of Medicinal Chemistry, 64(10), 6745-6764.

Demuro, S., Di Martino, R. M., Ortega, J. A., & Cavalli, A. (2021). GSK-3β, FYN, and DYRK1A: master regulators in neurodegenerative pathways. International Journal of Molecular Sciences, 22(16), 9098.

Laham, A. J., Saber-Ayad, M., & El-Awady, R. (2021). DYRK1A: A down syndrome-related dual protein kinase with a versatile role in tumorigenesis. Cellular and Molecular Life Sciences, 78(2), 603-619.

Bhansali, R. S., Rammohan, M., Lee, P., Laurent, A. P., Wen, Q., Suraneni, P., ... & Crispino, J. D. (2021). DYRK1A regulates B cell acute lymphoblastic leukemia through phosphorylation of FOXO1 and STAT3. The Journal of clinical investigation, 131(1).

Lee Walmsley, D., Murray, J. B., Dokurno, P., Massey, A. J., Benwell, K., Fiumana, A., ... & Hubbard, R. E. (2021). Fragment-derived selective inhibitors of dual-specificity kinases DYRK1A and DYRK1B. Journal of Medicinal Chemistry, 64(13), 8971-8991.

Walter, C., Marada, A., Suhm, T., Ernsberger, R., Muders, V., Kücükköse, C., ... & Meisinger, C. (2021). Global kinome profiling reveals DYRK1A as critical activator of the human mitochondrial import machinery. Nature communications, 12(1), 1-12.

Kumar, K., Suebsuwong, C., Wang, P., Garcia-Ocana, A., Stewart, A. F., & DeVita, R. J. (2021). DYRK1A inhibitors as potential therapeutics for β-cell regeneration for diabetes. Journal of Medicinal Chemistry, 64(6), 2901-2922.

Recasens, A., Humphrey, S. J., Ellis, M., Hoque, M., Abbassi, R. H., Chen, B., ... & Munoz, L. (2021). Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells. Cell death discovery, 7(1), 1-16.

Arbones, M. L., Thomazeau, A., Nakano-Kobayashi, A., Hagiwara, M., & Delabar, J. M. (2019). DYRK1A and cognition: A lifelong relationship. Pharmacology & therapeutics, 194, 199-221.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-DYRK1A Recombinant Antibody (CBYCD-463) (CAT#: CBMAB-D2016-YC)

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Isotype control

For research use only. Not intended for any clinical use.

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