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  • Rabbit Anti-DYRK1A Recombinant Antibody (EG1014)

Rabbit Anti-DYRK1A Recombinant Antibody (EG1014) (V2LY-0425-LY1152)

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Published Data
Rabbit Anti-DYRK1A Recombinant Antibody (EG1014) (V2LY-0425-LY1152-1) in WB
Rabbit Anti-DYRK1A Recombinant Antibody (EG1014) (V2LY-0425-LY1152-1) in WB
Western blots showing DYRK1A expression in HCT-116, SUM-159 and MDA-MB-231cells silenced or not with various CRISPR gRNAs. ...View More
Laham, A. J., El-Awady, R., Saber-Ayad, M., Wang, N., Yan, G., Boudreault, J., ... & Lebrun, J. J. (2024). Targeting the DYRK1A kinase prevents cancer progression and metastasis and promotes cancer cells response to G1/S targeting chemotherapy drugs. NPJ Precision Oncology, 8(1), 128.
Rabbit Anti-DYRK1A Recombinant Antibody (EG1014) (V2LY-0425-LY1152-2) in IP
Rabbit Anti-DYRK1A Recombinant Antibody (EG1014) (V2LY-0425-LY1152-2) in IP
Coimmunoprecipitation of IRS1myc in HEK293T cells with DYRK1A-GFP. ...View More
Frendo-Cumbo, S., Li, T., Ammendolia, D. A., Coyaud, E., Laurent, E. M., Liu, Y., ... & Brumell, J. H. (2022). DCAF7 regulates cell proliferation through IRS1-FOXO1 signaling. Iscience, 25(10).
Rabbit Anti-DYRK1A Recombinant Antibody (EG1014) (V2LY-0425-LY1152-3) in WB
Rabbit Anti-DYRK1A Recombinant Antibody (EG1014) (V2LY-0425-LY1152-3) in WB
C2C12 NT1 and Δwdr68-9 sublines in growth medium (GM, lanes 1-2) versus differentiation medium (DM, lanes 3-4). WDR68 panel: Lanes 1 and 3, WDR68 was detected in NT1 cells and induced higher in DM. Lanes 2 and 4, WDR68 was not detected above background in Δwdr68-9 cells. DYRK1A panel: Lanes 1 and 3, DYRK1A was detected in NT1 cells and induced higher in DM. Lanes 2 and 4, reduced DYRK1A expression in Δwdr68-9. β-tubulin panel: β-tubulin controls indicated similar loading in each lane. A') Quantitative analysis confirmed significantly reduced levels of WDR68 in the Δwdr68-9 subline. Three independent biological replicates were analyzed. A") Quantitative analysis confirmed significantly reduced DYRK1A expression in the Δwdr68-9 subline. Four independent biological replicates were analyzed. ...View More
Yousefelahiyeh, M., Xu, J., Alvarado, E., Yu, Y., Salven, D., & Nissen, R. M. (2018). DCAF7/WDR68 is required for normal levels of DYRK1A and DYRK1B. PLoS One, 13(11), e0207779.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Rabbit
Clone
EG1014
Application
WB, IP
Immunogen
Synthetic peptide corresponding to residues surrounding Asn523 of human DYRK1A protein.
Host Species
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:1,000
IP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & HEPES
Preservative
Sodium Azide
Concentration
Batch dependent
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1A
Entrez Gene ID
Human1859
Mouse13548
Rat25255
UniProt ID
HumanQ13627
MouseQ61214
RatQ63470
Research Area
Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. May play a role in a signaling pathway regulating nuclear functions of cell proliferation. Modulates alternative splicing by phosphorylating the splice factor SRSF6 (By similarity).

Exhibits a substrate preference for proline at position P+1 and arginine at position P-3. Has pro-survival function and negatively regulates the apoptotic process. Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1. This in turn inhibits TP53 activity and apoptosis (By similarity).
Biological Process
Amyloid-beta formation Source: ARUK-UCL
Circadian rhythm Source: UniProtKB
Negative regulation of DNA damage response, signal transduction by p53 class mediator Source: BHF-UCL
Negative regulation of microtubule polymerization Source: ARUK-UCL
Negative regulation of mRNA splicing, via spliceosome Source: Ensembl
Nervous system development Source: ProtInc
Peptidyl-serine autophosphorylation Source: ARUK-UCL
Peptidyl-serine phosphorylation Source: ARUK-UCL
Peptidyl-threonine phosphorylation Source: BHF-UCL
Peptidyl-tyrosine autophosphorylation Source: ARUK-UCL
Peptidyl-tyrosine phosphorylation Source: MGI
Positive regulation of protein deacetylation Source: BHF-UCL
Positive regulation of RNA splicing Source: ARUK-UCL
Positive regulation of transcription, DNA-templated Source: GO_Central
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: UniProtKB
Regulation of alternative mRNA splicing, via spliceosome Source: Ensembl
Cellular Location
Nucleus; Nucleus speckle
Involvement in disease
Mental retardation, autosomal dominant 7 (MRD7):
A disease characterized by primary microcephaly, severe mental retardation without speech, anxious autistic behavior, and dysmorphic features, including bitemporal narrowing, deep-set eyes, large simple ears, and a pointed nasal tip. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period.
PTM
Autophosphorylated on numerous tyrosine residues. Can also autophosphorylate on serine and threonine residues (in vitro).

Bessone, I. F., Navarro, J., Martinez, E., Karmirian, K., Holubiec, M., Alloatti, M., ... & Falzone, T. L. (2022). DYRK1A regulates the bidirectional axonal transport of APP in human-derived neurons. Journal of Neuroscience, 42(33), 6344-6358.

Weber, C., Sipos, M., Paczal, A., Balint, B., Kun, V., Foloppe, N., ... & Kotschy, A. (2021). Structure-guided discovery of potent and selective DYRK1A inhibitors. Journal of Medicinal Chemistry, 64(10), 6745-6764.

Demuro, S., Di Martino, R. M., Ortega, J. A., & Cavalli, A. (2021). GSK-3β, FYN, and DYRK1A: master regulators in neurodegenerative pathways. International Journal of Molecular Sciences, 22(16), 9098.

Laham, A. J., Saber-Ayad, M., & El-Awady, R. (2021). DYRK1A: A down syndrome-related dual protein kinase with a versatile role in tumorigenesis. Cellular and Molecular Life Sciences, 78(2), 603-619.

Bhansali, R. S., Rammohan, M., Lee, P., Laurent, A. P., Wen, Q., Suraneni, P., ... & Crispino, J. D. (2021). DYRK1A regulates B cell acute lymphoblastic leukemia through phosphorylation of FOXO1 and STAT3. The Journal of clinical investigation, 131(1).

Lee Walmsley, D., Murray, J. B., Dokurno, P., Massey, A. J., Benwell, K., Fiumana, A., ... & Hubbard, R. E. (2021). Fragment-derived selective inhibitors of dual-specificity kinases DYRK1A and DYRK1B. Journal of Medicinal Chemistry, 64(13), 8971-8991.

Walter, C., Marada, A., Suhm, T., Ernsberger, R., Muders, V., Kücükköse, C., ... & Meisinger, C. (2021). Global kinome profiling reveals DYRK1A as critical activator of the human mitochondrial import machinery. Nature communications, 12(1), 1-12.

Kumar, K., Suebsuwong, C., Wang, P., Garcia-Ocana, A., Stewart, A. F., & DeVita, R. J. (2021). DYRK1A inhibitors as potential therapeutics for β-cell regeneration for diabetes. Journal of Medicinal Chemistry, 64(6), 2901-2922.

Recasens, A., Humphrey, S. J., Ellis, M., Hoque, M., Abbassi, R. H., Chen, B., ... & Munoz, L. (2021). Global phosphoproteomics reveals DYRK1A regulates CDK1 activity in glioblastoma cells. Cell death discovery, 7(1), 1-16.

Arbones, M. L., Thomazeau, A., Nakano-Kobayashi, A., Hagiwara, M., & Delabar, J. M. (2019). DYRK1A and cognition: A lifelong relationship. Pharmacology & therapeutics, 194, 199-221.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Rabbit Anti-DYRK1A Recombinant Antibody (EG1014) (CAT#: CBMAB-EN1197-LY)

Related Products

Mouse Anti-DYRK1A Recombinant Antibody (CBYCD-463) (CAT#: CBMAB-D2016-YC)

Rabbit Anti-DYRK1A Recombinant Antibody (EG1014) (CAT#: CBMAB-EN1197-LY)

Mouse Anti-DYRK1A Recombinant Antibody (CBYCD-463) (CAT#: V2LY-0425-LY1151)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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