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Mouse Anti-E2F3 Recombinant Antibody (5F7) (CBMAB-A2415-LY)

The product is antibody recognizes E2F3. The antibody 5F7 immunoassay techniques such as: sELISA, ELISA.
See all E2F3 antibodies
Published Data
Fig.1 MiR-377 targets E2F3.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
5F7
Antibody Isotype
IgG2b, κ
Application
sELISA, ELISA

Basic Information

Immunogen
E2F3 (AAH16847.1, 1 a.a. ~ 133 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
E2F transcription factor 3
Introduction
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq]
Entrez Gene ID
1871
UniProt ID
O00716
Alternative Names
DKFZp686C18211; E2F-3; KIAA0075; MGC104598
Research Area
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner. Inhibits adipogenesis, probably through the repression of CEBPA binding to its target gene promoters (By similarity).
Biological Process
G1/S transition of mitotic cell cycle Source: UniProtKB
Negative regulation of fat cell proliferation Source: UniProtKB
Positive regulation of cell population proliferation Source: Ensembl
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of vascular associated smooth muscle cell apoptotic process Source: BHF-UCL
Protein import into nucleus Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Transcription initiation from RNA polymerase II promoter Source: ProtInc
Cellular Location
Nucleus

Yan, X., Wang, T., & Wang, J. (2022). Circ_0016760 acts as a sponge of microRNA-4295 to enhance E2F transcription factor 3 expression and facilitates cell proliferation and glycolysis in non-small cell lung cancer. Cancer Biotherapy & Radiopharmaceuticals, 37(2), 147-158.

Wu, X., Kong, C., & Wu, Y. (2021). Long intergenic non-protein coding RNA 1094 (LINC01094) promotes the progression of breast cancer (BC) by regulating the microRNA-340-5p (miR-340-5p)/E2F transcription factor 3 (E2F3) axis. Bioengineered, 12(1), 9046-9057.

Li, H., Pan, R., Lu, Q., Ren, C., Sun, J., Wu, H., ... & Chen, H. (2020). MicroRNA‑145‑5p inhibits osteosarcoma cell proliferation by targeting E2F transcription factor 3. International journal of molecular medicine, 45(5), 1317-1326.

Zhao, W., Wang, S., Qin, T., & Wang, W. (2020). Circular RNA (circ‐0075804) promotes the proliferation of retinoblastoma via combining heterogeneous nuclear ribonucleoprotein K (HNRNPK) to improve the stability of E2F transcription factor 3 E2F3. Journal of Cellular Biochemistry, 121(7), 3516-3525.

Shi, L., Zhu, H., Shen, Y., Dou, X., Guo, H., Wang, P., ... & Zou, X. (2020). Regulation of E2F transcription factor 3 by microRNA-152 modulates gastric cancer invasion and metastasis. Cancer management and research, 12, 1187.

Santerre, M., Bagashev, A., Gorecki, L., Lysek, K. Z., Wang, Y., Shrestha, J., ... & Sawaya, B. E. (2019). HIV-1 Tat protein promotes neuronal dysregulation by inhibiting E2F transcription factor 3 (E2F3). Journal of Biological Chemistry, 294(10), 3618-3633.

Li, Q., Pan, H., & Liu, Q. (2019). MicroRNA-15a modulates lens epithelial cells apoptosis and proliferation through targeting B-cell lymphoma-2 and E2F transcription factor 3 in age-related cataracts. Bioscience Reports, 39(12).

Liu, N., Liu, Z., Zhang, W., Li, Y., Cao, J., Yang, H., & Li, X. (2018). MicroRNA‑433 reduces cell proliferation and invasion in non‑small cell lung cancer via directly targeting E2F transcription factor 3. Molecular Medicine Reports, 18(1), 1155-1164.

Gao, X., Zhao, S., Yang, X., Zang, S., & Yuan, X. (2018). Long non-coding RNA FLVCR1-AS1 contributes to the proliferation and invasion of lung cancer by sponging miR-573 to upregulate the expression of E2F transcription factor 3. Biochemical and biophysical research communications, 505(3), 931-938.

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For research use only. Not intended for any clinical use.

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