Mouse Anti-E2F4 Recombinant Antibody (CBFYE-0005) (V2LY-0425-LY1240)

Name: Mouse Anti-E2F4 Recombinant Antibody (CBFYE-0005)
SKU: V2LY-0425-LY1240
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-E2F4 Recombinant Antibody (CBFYE-0005) (V2LY-0425-LY1240-1) in WB

Cytoplasmic and nuclear E2F4 and E2F5 were examined in GBM cells by Western blot in unstimulated vs stimulated conditions (10 ng/mL TWEAK for 4 h) with RelB as a positive TWEAK-inducible control and Lamin A as a nuclear marker. ...View More

Pflug, K. M., Lee, D. W., McFadden, K., Herrera, L., & Sitcheran, R. (2023). Transcriptional induction of NF-κB-inducing kinase by E2F4/5 facilitates collective invasion of GBM cells. Scientific Reports, 13(1), 13093.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0005

Application

WB, IP, IF, IHC-P

Immunogen

Full length of recombinant human E2F-4.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

E2F4

Entrez Gene ID

Human	1874
Mouse	104394
Rat	100360427

UniProt ID

Human	Q16254
Mouse	Q8R0K9
Rat	D4A9V4

Research Area

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.

Biological Process

Animal organ morphogenesis Source: Ensembl
Blood circulation Source: Ensembl
Cell volume homeostasis Source: Ensembl
Centriole assembly Source: UniProtKB
Epithelial cell development Source: Ensembl
Motile cilium assembly Source: UniProtKB
Multi-ciliated epithelial cell differentiation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of cell population proliferation Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl

Cellular Location

Nucleus

PTM

Differentially phosphorylated in vivo.

Dong, C., Yue, X., Zhu, L., Ge, P., Zheng, G., Ye, Z., & Pan, B. (2022). Curcumin Attenuates Hydrocephalus via Activation of E2F Transcription Factor 4. Cerebrovascular Diseases, 1-8.

Xiao, W., Wang, J., Wang, X., Cai, S., Guo, Y., Ye, L., ... & Zheng, L. (2022). Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression. Autophagy, 1-21.

Chen, P., Wang, L., Long, Y. B., Liang, G. Y., Yang, X., Dong, Z. Q., ... & Lu, C. (2022). E2F4 regulates the cell cycle and DNA replication in the silkworm, Bombyx mori. Insect Science, 29(4), 1006-1016.

Wei, J., Shi, Y., Zou, C., Zhang, H., Peng, H., Wang, S., ... & Wang, D. (2022). Cellular Id1 inhibits hepatitis B virus transcription by interacting with the novel covalently closed circular DNA-binding protein E2F4. International journal of biological sciences, 18(1), 65.

Zheng, Q., Fu, Q., Xu, J., Gu, X., Zhou, H., & Zhi, C. (2021). Transcription factor E2F4 is an indicator of poor prognosis and is related to immune infiltration in hepatocellular carcinoma. Journal of Cancer, 12(6), 1792.

Liu, J., Xia, L., Wang, S., Cai, X., Wu, X., Zou, C., ... & Wang, D. (2021). E2F4 Promotes the Proliferation of Hepatocellular Carcinoma Cells through Upregulation of CDCA3. Journal of Cancer, 12(17), 5173.

Kim, J., Yim, G. W., Lee, D. W., Kim, Y. T., Lee, Y. J., & Rhee, Y. J. (2021). Knockdown of E2F4 suppresses the growth of ovarian cancer cells through the cell cycle pathway. International Journal of Clinical and Experimental Pathology, 14(8), 866.

Hong, J., Wang, X., Mei, C., & Zan, L. (2019). Competitive regulation by transcription factors and DNA methylation in the bovine SIRT5 promoter: Roles of E2F4 and KLF6. Gene, 684, 39-46.

Hsu, J., Arand, J., Chaikovsky, A., Mooney, N. A., Demeter, J., Brison, C. M., ... & Sage, J. (2019). E2F4 regulates transcriptional activation in mouse embryonic stem cells independently of the RB family. Nature communications, 10(1), 1-14.

Bhawe, K., & Roy, D. (2018). Interplay between NRF1, E2F4 and MYC transcription factors regulating common target genes contributes to cancer development and progression. Cellular Oncology, 41(5), 465-484.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-E2F4 (AA 211-301) Recombinant Antibody (CBFYE-0005) (CAT#: CBMAB-E0012-FY)

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Isotype control

For research use only. Not intended for any clinical use.

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