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Mouse Anti-E2F4 Recombinant Antibody (CBFYE-0005) (V2LY-0425-LY1240)


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Published Data
Mouse Anti-E2F4 Recombinant Antibody (CBFYE-0005) (V2LY-0425-LY1240-1) in WB
Mouse Anti-E2F4 Recombinant Antibody (CBFYE-0005) (V2LY-0425-LY1240-1) in WB
Cytoplasmic and nuclear E2F4 and E2F5 were examined in GBM cells by Western blot in unstimulated vs stimulated conditions (10 ng/mL TWEAK for 4 h) with RelB as a positive TWEAK-inducible control and Lamin A as a nuclear marker. ...View More
Pflug, K. M., Lee, D. W., McFadden, K., Herrera, L., & Sitcheran, R. (2023). Transcriptional induction of NF-κB-inducing kinase by E2F4/5 facilitates collective invasion of GBM cells. Scientific Reports, 13(1), 13093.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYE-0005
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, IHC-P

Basic Information

Immunogen
Full length of recombinant human E2F-4.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
E2F4
Entrez Gene ID
Human1874
Mouse104394
Rat100360427
UniProt ID
HumanQ16254
MouseQ8R0K9
RatD4A9V4
Research Area
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
Biological Process
Animal organ morphogenesis Source: Ensembl
Blood circulation Source: Ensembl
Cell volume homeostasis Source: Ensembl
Centriole assembly Source: UniProtKB
Epithelial cell development Source: Ensembl
Motile cilium assembly Source: UniProtKB
Multi-ciliated epithelial cell differentiation Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Regulation of cell population proliferation Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Ensembl
Cellular Location
Nucleus
PTM
Differentially phosphorylated in vivo.
More Infomation

Dong, C., Yue, X., Zhu, L., Ge, P., Zheng, G., Ye, Z., & Pan, B. (2022). Curcumin Attenuates Hydrocephalus via Activation of E2F Transcription Factor 4. Cerebrovascular Diseases, 1-8.

Xiao, W., Wang, J., Wang, X., Cai, S., Guo, Y., Ye, L., ... & Zheng, L. (2022). Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression. Autophagy, 1-21.

Chen, P., Wang, L., Long, Y. B., Liang, G. Y., Yang, X., Dong, Z. Q., ... & Lu, C. (2022). E2F4 regulates the cell cycle and DNA replication in the silkworm, Bombyx mori. Insect Science, 29(4), 1006-1016.

Wei, J., Shi, Y., Zou, C., Zhang, H., Peng, H., Wang, S., ... & Wang, D. (2022). Cellular Id1 inhibits hepatitis B virus transcription by interacting with the novel covalently closed circular DNA-binding protein E2F4. International journal of biological sciences, 18(1), 65.

Zheng, Q., Fu, Q., Xu, J., Gu, X., Zhou, H., & Zhi, C. (2021). Transcription factor E2F4 is an indicator of poor prognosis and is related to immune infiltration in hepatocellular carcinoma. Journal of Cancer, 12(6), 1792.

Liu, J., Xia, L., Wang, S., Cai, X., Wu, X., Zou, C., ... & Wang, D. (2021). E2F4 Promotes the Proliferation of Hepatocellular Carcinoma Cells through Upregulation of CDCA3. Journal of Cancer, 12(17), 5173.

Kim, J., Yim, G. W., Lee, D. W., Kim, Y. T., Lee, Y. J., & Rhee, Y. J. (2021). Knockdown of E2F4 suppresses the growth of ovarian cancer cells through the cell cycle pathway. International Journal of Clinical and Experimental Pathology, 14(8), 866.

Hong, J., Wang, X., Mei, C., & Zan, L. (2019). Competitive regulation by transcription factors and DNA methylation in the bovine SIRT5 promoter: Roles of E2F4 and KLF6. Gene, 684, 39-46.

Hsu, J., Arand, J., Chaikovsky, A., Mooney, N. A., Demeter, J., Brison, C. M., ... & Sage, J. (2019). E2F4 regulates transcriptional activation in mouse embryonic stem cells independently of the RB family. Nature communications, 10(1), 1-14.

Bhawe, K., & Roy, D. (2018). Interplay between NRF1, E2F4 and MYC transcription factors regulating common target genes contributes to cancer development and progression. Cellular Oncology, 41(5), 465-484.

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For research use only. Not intended for any clinical use.

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