Mouse Anti-EAF1 Recombinant Antibody (1G2) (CBMAB-A2421-LY)

The product is antibody recognizes EAF1. The antibody 1G2 immunoassay techniques such as: WB, ELISA.
See all EAF1 antibodies

Summary

Host Animal

Mouse

Specificity

Human

Clone

1G2

Antibody Isotype

IgG2a, κ

Application

WB, ELISA

Basic Information

Immunogen

EAF1 (AAH41329, 1 a.a. ~ 268 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

ELL associated factor 1

Entrez Gene ID

85403

UniProt ID

Q96JC9

Research Area

Acts as a transcriptional transactivator of ELL and ELL2 elongation activities.

Biological Process

Regulation of transcription elongation from RNA polymerase II promoter Source: ARUK-UCL

Cellular Location

Nucleus speckle; Cajal body

References

Basu, S., Nandy, A., Barad, M. K., Pal, S., & Biswas, D. (2022). Negative Feedback Loop Mechanism between EAF1/2 and DBC1 in Regulating ELL Stability and Functions. Molecular and Cellular Biology, e00151-22.

Liu, R., Chen, C., Li, Y., Huang, Q., & Xue, Y. (2020). ELL-associated factors EAF1/2 negatively regulate HIV-1 transcription through inhibition of Super Elongation Complex formation. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1863(5), 194508.

Pascal, L. E., Su, F., Wang, D., Ai, J., Song, Q., Wang, Y., ... & Wang, Z. (2019). Conditional deletion of eaf1 induces murine prostatic intraepithelial neoplasia in mice. Neoplasia, 21(8), 752-764.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-EAF1 Recombinant Antibody (CBFYE-0011)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
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Second antibody and isotype control

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