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Mouse Anti-EAF1 Recombinant Antibody (CBFYE-0011) (V2LY-0425-LY1262)


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Published Data
Mouse Anti-EAF1 Recombinant Antibody (CBFYE-0011) (V2LY-0425-LY1262-1) in WB and IP
Mouse Anti-EAF1 Recombinant Antibody (CBFYE-0011) (V2LY-0425-LY1262-1) in WB and IP
Western blotting for immunoprecipitates copurified with FLAG-tagged MED26 (F-MED26) by anti-FLAG antibody. F-MED26 was stably expressed in HEK293T cells expressing wild-type EAF1 (wt) or EAF1-mutant (mut). Anti-FLAG-immunopurification was performed and the resulting immunoprecipitates were subjected to western blotting. ...View More
Suzuki, H., Abe, R., Shimada, M., Hirose, T., Hirose, H., Noguchi, K., ... & Takahashi, H. (2022). The 3' Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies' association with histone locus bodies. Nature communications, 13(1), 2905.
Mouse Anti-EAF1 Recombinant Antibody (CBFYE-0011) (V2LY-0425-LY1262-2) in IF
Mouse Anti-EAF1 Recombinant Antibody (CBFYE-0011) (V2LY-0425-LY1262-2) in IF
Super-resolution images showing relative positions of HLBs, Mediator, and CBs. Wild-type cells (WT, upper) and EAF1-mutant cells (EAF1 mut, lower) were fixed with paraformaldehyde, and triple immunofluorescence staining for NPAT (green), MED26 (red), and Coilin (gray) was performed. Enlarged images for representative particles are shown in the lower part of each image. ...View More
Suzuki, H., Abe, R., Shimada, M., Hirose, T., Hirose, H., Noguchi, K., ... & Takahashi, H. (2022). The 3' Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies' association with histone locus bodies. Nature communications, 13(1), 2905.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Dog, Cattle
Clone
CBFYE-0011
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 2-29 at the N-terminus of human EAF1.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Dog, Cattle
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
ELL associated factor 1
Entrez Gene ID
85403
UniProt ID
Q96JC9
Research Area
Acts as a transcriptional transactivator of ELL and ELL2 elongation activities.
Biological Process
Regulation of transcription elongation from RNA polymerase II promoter Source: ARUK-UCL
Cellular Location
Nucleus speckle; Cajal body
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For research use only. Not intended for any clinical use.

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