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Mouse Anti-EBF1 Recombinant Antibody (1G8) (CBMAB-A2424-LY)

The product is antibody recognizes EBF1. The antibody 1G8 immunoassay techniques such as: WB, ELISA.
See all EBF1 antibodies
Published Data
Fig.1 EBF1 interacts with TET2 at hypermethylated loci.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
1G8
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
EBF1 (AAH38805, 1 a.a. ~ 591 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
EBF1
Entrez Gene ID
1879
UniProt ID
Q9UH73
Alternative Names
COE1; EBF; FLJ39389; FLJ41763; O/E-1; OLF1
Research Area
Key pioneer transcription factor of B-cell specification and commitment (PubMed:27807034).

Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).

(Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed:28968461).

In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed:26819314).
Biological Process
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus

Shuang, O., Zhou, J., Cai, Z., Liao, L., Wang, Y., Wang, W., & Xu, M. (2022). EBF1-mediated up-regulation of lncRNA FGD5-AS1 facilitates osteosarcoma progression by regulating miR-124-3p/G3BP2 axis as a ceRNA. Journal of Orthopaedic Surgery and Research, 17(1), 1-14.

Qiu, Z., Guo, W., Dong, B., Wang, Y., Deng, P., Wang, C., ... & Wu, Y. (2022). EBF1 promotes triple-negative breast cancer progression by surveillance of the HIF1α pathway. Proceedings of the National Academy of Sciences, 119(28), e2119518119.

Pagani, F., Tratta, E., Dell’Era, P., Cominelli, M., & Poliani, P. L. (2021). EBF1 is expressed in pericytes and contributes to pericyte cell commitment. Histochemistry and cell biology, 156(4), 333-347.

El-Magd, M. A., Abdelfattah-Hassan, A., Elsisy, R. A., Hawsawi, Y. M., Oyouni, A. A., Al-Amer, O. M., & El-Shetry, E. S. (2021). Expression and function of Ebf1 gene during chondrogenesis in chick embryo limb buds. Gene, 803, 145895.

Somasundaram, R., Jensen, C. T., Tingvall-Gustafsson, J., Åhsberg, J., Okuyama, K., Prasad, M., ... & Sigvardsson, M. (2021). EBF1 and PAX5 control pro-B cell expansion via opposing regulation of the Myc gene. Blood, 137(22), 3037-3049.

Luo, H., Yang, L., Liu, C., Wang, X., Dong, Q., Liu, L., & Wei, Q. (2020). TMPO-AS1/miR-98-5p/EBF1 feedback loop contributes to the progression of bladder cancer. The International Journal of Biochemistry & Cell Biology, 122, 105702.

Wang, Y., Zolotarev, N., Yang, C. Y., Rambold, A., Mittler, G., & Grosschedl, R. (2020). A prion-like domain in transcription factor EBF1 promotes phase separation and enables B cell programming of progenitor chromatin. Immunity, 53(6), 1151-1167.

Nelson, T., Velazquez, H., Troiano, N., & Fretz, J. A. (2019). Early B cell factor 1 (EBF1) regulates glomerular development by controlling mesangial maturation and consequently COX-2 expression. Journal of the American Society of Nephrology, 30(9), 1559-1572.

Boller, S., Li, R., & Grosschedl, R. (2018). Defining B cell chromatin: lessons from EBF1. Trends in Genetics, 34(4), 257-269.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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