EBF1 Antibodies

Structure of EBF1

The protein encoded by the EBF1 gene has a molecular weight of approximately 68 kDa, and there is a slight variation due to differences in sequence length among different species. The human EBF1 consists of 591 amino acids and contains a conserved DNA-binding domain and an HLH dimerization domain.

This protein binds to the E-box-like motifs in DNA by forming homodimers or heterodimers. The N-terminal DBD domain adopts an immunoglobulin-like folding pattern, the central HLH region mediates dimerization, and the C-terminal transcriptional activation domain is rich in serine and proline residues. EBF1 recognizes the palindromic structure of the sequence 5'-ATTCCC(N)3-4GGGAAT-3'. When the dimer embeds in the major groove of DNA, it causes a local curvature of approximately 35°. Chicken embryo experiments have shown that its DNA binding activity is cooperatively regulated by Zn²⁺ions.

Fig. 1 Summary of the regulatory mechanisms of EBF1 in the initiation and progression of BC.¹

Key structural properties of EBF1:

• The HLH domain mediates protein dimerization
• The zinc finger-like DNA binding domain recognizes palindromic sequences
• The transcriptional activation region is rich in Ser/Pro residues
• The PTD domain is involved in B-cell development regulation
• Homodimer/heterodimer cooperatively binds to target sites

Functions of EBF1

The core function of EBF1 is to regulate the early differentiation of B cells and the generation of adipocytes. Additionally, it also plays a role in neural development, osteogenesis processes, and metabolic regulation.

The expression of EBF1 in B cells shows a dynamic change: it is silent during the hematopoietic stem cell stage, activated during the lymphoid progenitor cell stage, and maintains a constant level in mature B cells; once its expression is lost, the development of B cells will completely stall.

Applications of EBF1 and EBF1 Antibody in Literature

1. Li, Qinghua, et al. "Advances in Research on the B-Lineage Transcription Factor EBF1 in Solid Tumors." International Journal of Molecular Sciences 26.11 (2025): 5203. https://doi.org/10.3390/ijms26115203

The article indicates that the early B-cell factor 1 (EBF1) is a key transcription factor regulating B-cell development. Its role in solid tumors is complex and diverse, and its function can change according to the type of tumor and the microenvironment, being closely related to tumor occurrence and development. This article reviews its multiple roles in solid tumors and the related pathway mechanisms.

2. Kim, Eugene E., et al. "The transcription factor EBF1 non-cell-autonomously regulates cardiac growth and differentiation." Development 150.21 (2023): dev202054. https://doi.org/10.1242/dev.202054

The article indicates that the transcription factor EBF1 is highly expressed in non-muscular cells of the heart. Its absence leads to abnormal proliferation of cardiac muscle cells, incomplete development of the conduction system, and accumulation of MYC protein.

3. Pagani, Francesca, et al. "EBF1 is expressed in pericytes and contributes to pericyte cell commitment." Histochemistry and cell biology 156.4 (2021): 333-347. https://doi.org/10.1007/s00418-021-02015-7

The article indicates that the transcription factor EBF1 is highly expressed in pericytes and specifically regulates the expression of its markers PDGFRβ and CD146. Silencing EBF1 reduces the expression of pericyte-related factors, suggesting that EBF1 determines the differentiation of cells into the pericyte phenotype.

4. Mesuraca, Maria, et al. "ZNF423 and ZNF521: EBF1 antagonists of potential relevance in B‐lymphoid malignancies." BioMed research international 2015.1 (2015): 165238. https://doi.org/10.1155/2015/165238

The article indicates that EBF1 is a key transcription factor regulating B-cell development, and its functional deficiency is associated with the occurrence of B-cell leukemia. The study reveals that zinc finger proteins ZNF423 and ZNF521, as strong inhibitors of EBF1, play a potentially important role in the development of leukemia.

5. Wu, Yueheng, et al. "Transcriptome analysis reveals EBF1 ablation-induced injuries in cardiac system." Theranostics 14.12 (2024): 4894. https://doi.org/10.7150/thno.92060

The article indicates that the transcription factor EBF1 is expressed in the heart. Its absence will inhibit heart development and cause myocardial hypertrophy. The study found that EBF1 regulates the transcriptional expression of genes related to heart development and hypertrophy by directly binding to the promoter regions of these genes, thereby influencing the process of heart remodeling.

Creative Biolabs: EBF1 Antibodies for Research

Creative Biolabs specializes in the production of high-quality EBF1 antibodies for research and industrial applications. Our portfolio includes monoclonal and polyclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom EBF1 Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our EBF1 antibodies, custom preparations, or technical support, contact us at info@creative-biolabs.com.