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Mouse Anti-EBF1 Recombinant Antibody (T26-818) (CBMAB-E0703-FY)

This product is mouse antibody that recognizes EBF1. The antibody T26-818 can be used for immunoassay techniques such as: FC.
See all EBF1 antibodies

Summary

Host Animal
Mouse
Specificity
Mouse
Clone
T26-818
Antibody Isotype
IgG1, κ
Application
FC

Basic Information

Specificity
Mouse
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
0.2 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
EBF1
Introduction
EBF1 overexpression results in plants insensitive to ethylene. In contrast, plants carrying the ebf1 and ebf2 mutations display a constitutive ethylene response and accumulate the EIN3 protein in the absence of the hormone.
Entrez Gene ID
13591
UniProt ID
Q5SWK4
Alternative Names
Ebf; OE-1; Olf1; O/E-1; Olf-1
Research Area
Key pioneer transcription factor of B-cell specification and commitment (PubMed:27807034).

Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).

(Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed:28968461).

In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed:26819314).
Biological Process
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus

Shuang, O., Zhou, J., Cai, Z., Liao, L., Wang, Y., Wang, W., & Xu, M. (2022). EBF1-mediated up-regulation of lncRNA FGD5-AS1 facilitates osteosarcoma progression by regulating miR-124-3p/G3BP2 axis as a ceRNA. Journal of Orthopaedic Surgery and Research, 17(1), 1-14.

Qiu, Z., Guo, W., Dong, B., Wang, Y., Deng, P., Wang, C., ... & Wu, Y. (2022). EBF1 promotes triple-negative breast cancer progression by surveillance of the HIF1α pathway. Proceedings of the National Academy of Sciences, 119(28), e2119518119.

Pagani, F., Tratta, E., Dell’Era, P., Cominelli, M., & Poliani, P. L. (2021). EBF1 is expressed in pericytes and contributes to pericyte cell commitment. Histochemistry and cell biology, 156(4), 333-347.

El-Magd, M. A., Abdelfattah-Hassan, A., Elsisy, R. A., Hawsawi, Y. M., Oyouni, A. A., Al-Amer, O. M., & El-Shetry, E. S. (2021). Expression and function of Ebf1 gene during chondrogenesis in chick embryo limb buds. Gene, 803, 145895.

Somasundaram, R., Jensen, C. T., Tingvall-Gustafsson, J., Åhsberg, J., Okuyama, K., Prasad, M., ... & Sigvardsson, M. (2021). EBF1 and PAX5 control pro-B cell expansion via opposing regulation of the Myc gene. Blood, 137(22), 3037-3049.

Luo, H., Yang, L., Liu, C., Wang, X., Dong, Q., Liu, L., & Wei, Q. (2020). TMPO-AS1/miR-98-5p/EBF1 feedback loop contributes to the progression of bladder cancer. The International Journal of Biochemistry & Cell Biology, 122, 105702.

Wang, Y., Zolotarev, N., Yang, C. Y., Rambold, A., Mittler, G., & Grosschedl, R. (2020). A prion-like domain in transcription factor EBF1 promotes phase separation and enables B cell programming of progenitor chromatin. Immunity, 53(6), 1151-1167.

Nelson, T., Velazquez, H., Troiano, N., & Fretz, J. A. (2019). Early B cell factor 1 (EBF1) regulates glomerular development by controlling mesangial maturation and consequently COX-2 expression. Journal of the American Society of Nephrology, 30(9), 1559-1572.

Boller, S., Li, R., & Grosschedl, R. (2018). Defining B cell chromatin: lessons from EBF1. Trends in Genetics, 34(4), 257-269.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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