Mouse Anti-EDEM1 Recombinant Antibody (CBFYE-0025) (V2LY-0425-LY1395)

Name: Mouse Anti-EDEM1 Recombinant Antibody (CBFYE-0025)
SKU: V2LY-0425-LY1395
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EDEM1 Recombinant Antibody (CBFYE-0025) (V2LY-0425-LY1395-1) in WB

Protein levels of EDEM, a misfolded glycoprotein-binding protein in MCF-7/KCR cells treated with AgNPs. ...View More

Gopisetty, M. K., Kovács, D., Igaz, N., Rónavári, A., Bélteky, P., Rázga, Z., ... & Kiricsi, M. (2019). Endoplasmic reticulum stress: major player in size-dependent inhibition of P-glycoprotein by silver nanoparticles in multidrug-resistant breast cancer cells. Journal of nanobiotechnology, 17, 1-15.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0025

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 623-651 at the C-terminus of human EDEM.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ER degradation enhancer, mannosidase alpha-like 1

Entrez Gene ID

9695

UniProt ID

Q92611

Research Area

Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.

Biological Process

Carbohydrate metabolic process Source: InterPro
Mannose trimming involved in glycoprotein ERAD pathway Source: ParkinsonsUK-UCL
Positive regulation of retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
Protein targeting to ER Source: UniProtKB
Response to unfolded protein Source: UniProtKB-KW
Trimming of terminal mannose on C branch Source: Ensembl
Ubiquitin-dependent ERAD pathway Source: UniProtKB
Ubiquitin-dependent glycoprotein ERAD pathway Source: ParkinsonsUK-UCL

Cellular Location

Endoplasmic reticulum membrane

Topology

Cytoplasmic: 1-4
Helical: 5-25
Lumenal: 26-657

Nowakowska-Gołacka, J., Czapiewska, J., Sominka, H., Sowa-Rogozińska, N., & Słomińska-Wojewódzka, M. (2021). EDEM1 Regulates Amyloid Precursor Protein (APP) Metabolism and Amyloid-β Production. International journal of molecular sciences, 23(1), 117.

Li, C., Pan, B., Liu, X., Qin, J., Wang, X., He, B., ... & Wang, S. (2021). Long intergenic non-coding RNA LINC00485 exerts tumor-suppressive activity by regulating miR-581/EDEM1 axis in colorectal cancer. Aging (Albany NY), 13(3), 3866.

Xu, L., Xu, Q., Dai, S., Jiao, C., Tang, Y., Xie, J., ... & Chen, X. (2021). lncRNA Xist regulates sevoflurane-induced social and emotional impairment by modulating miR-98-5p/EDEM1 signaling axis in neonatal mice. Molecular Therapy-Nucleic Acids, 24, 939-950.

George, G., Ninagawa, S., Yagi, H., Furukawa, J. I., Hashii, N., Ishii-Watabe, A., ... & Mori, K. (2021). Purified EDEM3 or EDEM1 alone produces determinant oligosaccharide structures from M8B in mammalian glycoprotein ERAD. Elife, 10.

Chiritoiu, M., Chiritoiu, G. N., Munteanu, C. V., Pastrama, F., Ivessa, N. E., & Petrescu, S. M. (2020). EDEM1 drives misfolded protein degradation via erad and exploits er-phagy as back-up mechanism when ERAD is impaired. International Journal of Molecular Sciences, 21(10), 3468.

Xu, L., Xu, Q., Dai, S., Zhang, W., Wu, H., Xu, F., ... & Chen, X. (2020). Suppression of lncRNA Xist Improves Sevoflurane-Induced Social and Emotional Impairment by Activating miR-98-5p/EDEM1 Signaling Axis in Neonatal Mice. Available at SSRN 3576749.

Zhang, H. M., Qiu, Y., Zhao, G., Wang, H., Chen, Y. T., Aghakeshmiri, S., ... & Yang, D. (2020). Cleavage and degradation of EDEM1 promotes coxsackievirus B3 replication via ATF6a‐mediated unfolded protein response signalling. Cellular Microbiology, 22(7), e13198.

Shenkman, M., Ron, E., Yehuda, R., Benyair, R., Khalaila, I., & Lederkremer, G. Z. (2018). Mannosidase activity of EDEM1 and EDEM2 depends on an unfolded state of their glycoprotein substrates. Communications Biology, 1(1), 1-11.

Papaioannou, A., Higa, A., Jégou, G., Jouan, F., Pineau, R., Saas, L., ... & Chevet, E. (2018). Alterations of EDEM 1 functions enhance ATF 6 pro‐survival signaling. The FEBS Journal, 285(22), 4146-4164.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-EDEM1 (AA 559-656) Recombinant Antibody (CBFYE-0025) (CAT#: CBMAB-E0052-FY)

Related Products

Mouse Anti-EDEM1 (AA 559-656) Recombinant Antibody (CBFYE-0025) (CAT#: CBMAB-E0052-FY)

Isotype control

For research use only. Not intended for any clinical use.

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