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Mouse Anti-EDEM1 (AA 559-656) Recombinant Antibody (CBFYE-0025) (CBMAB-E0052-FY)

This product is mouse antibody that recognizes EDEM1. The antibody CBFYE-0025 can be used for immunoassay techniques such as: ELISA, WB.
See all EDEM1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-0025
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Partial recombinant from EDEM1 with GST tag MW of the GST tag alone is 26 KDa
Specificity
Human
Antibody Isotype
IgG2a, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 559-656

Target

Full Name
ER degradation enhancer, mannosidase alpha-like 1
Introduction
Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L.
Entrez Gene ID
9695
UniProt ID
Q92611
Alternative Names
EDEM
Research Area
Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.
Biological Process
Carbohydrate metabolic process Source: InterPro
Mannose trimming involved in glycoprotein ERAD pathway Source: ParkinsonsUK-UCL
Positive regulation of retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
Protein targeting to ER Source: UniProtKB
Response to unfolded protein Source: UniProtKB-KW
Trimming of terminal mannose on C branch Source: Ensembl
Ubiquitin-dependent ERAD pathway Source: UniProtKB
Ubiquitin-dependent glycoprotein ERAD pathway Source: ParkinsonsUK-UCL
Cellular Location
Endoplasmic reticulum membrane
Topology
Cytoplasmic: 1-4
Helical: 5-25
Lumenal: 26-657

Nowakowska-Gołacka, J., Czapiewska, J., Sominka, H., Sowa-Rogozińska, N., & Słomińska-Wojewódzka, M. (2021). EDEM1 Regulates Amyloid Precursor Protein (APP) Metabolism and Amyloid-β Production. International journal of molecular sciences, 23(1), 117.

Li, C., Pan, B., Liu, X., Qin, J., Wang, X., He, B., ... & Wang, S. (2021). Long intergenic non-coding RNA LINC00485 exerts tumor-suppressive activity by regulating miR-581/EDEM1 axis in colorectal cancer. Aging (Albany NY), 13(3), 3866.

Xu, L., Xu, Q., Dai, S., Jiao, C., Tang, Y., Xie, J., ... & Chen, X. (2021). lncRNA Xist regulates sevoflurane-induced social and emotional impairment by modulating miR-98-5p/EDEM1 signaling axis in neonatal mice. Molecular Therapy-Nucleic Acids, 24, 939-950.

George, G., Ninagawa, S., Yagi, H., Furukawa, J. I., Hashii, N., Ishii-Watabe, A., ... & Mori, K. (2021). Purified EDEM3 or EDEM1 alone produces determinant oligosaccharide structures from M8B in mammalian glycoprotein ERAD. Elife, 10.

Chiritoiu, M., Chiritoiu, G. N., Munteanu, C. V., Pastrama, F., Ivessa, N. E., & Petrescu, S. M. (2020). EDEM1 drives misfolded protein degradation via erad and exploits er-phagy as back-up mechanism when ERAD is impaired. International Journal of Molecular Sciences, 21(10), 3468.

Xu, L., Xu, Q., Dai, S., Zhang, W., Wu, H., Xu, F., ... & Chen, X. (2020). Suppression of lncRNA Xist Improves Sevoflurane-Induced Social and Emotional Impairment by Activating miR-98-5p/EDEM1 Signaling Axis in Neonatal Mice. Available at SSRN 3576749.

Zhang, H. M., Qiu, Y., Zhao, G., Wang, H., Chen, Y. T., Aghakeshmiri, S., ... & Yang, D. (2020). Cleavage and degradation of EDEM1 promotes coxsackievirus B3 replication via ATF6a‐mediated unfolded protein response signalling. Cellular Microbiology, 22(7), e13198.

Shenkman, M., Ron, E., Yehuda, R., Benyair, R., Khalaila, I., & Lederkremer, G. Z. (2018). Mannosidase activity of EDEM1 and EDEM2 depends on an unfolded state of their glycoprotein substrates. Communications Biology, 1(1), 1-11.

Papaioannou, A., Higa, A., Jégou, G., Jouan, F., Pineau, R., Saas, L., ... & Chevet, E. (2018). Alterations of EDEM 1 functions enhance ATF 6 pro‐survival signaling. The FEBS Journal, 285(22), 4146-4164.

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For research use only. Not intended for any clinical use.

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