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Mouse Anti-EEF1D Recombinant Antibody (CF143) (CBMAB-FT337LY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CF143
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 1-153 mapping at the N-terminus of human EF-1 δ.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG3, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Eukaryotic Translation Elongation Factor 1 Delta
Introduction
This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.[provided by RefSeq, Aug 2010]
Entrez Gene ID
Human1936
Mouse66656
Rat300033
UniProt ID
HumanP29692
MouseP57776
RatQ68FR9
Alternative Names
EF1D
Research Area
Isoform 1:
EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.

Isoform 2:
Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).
Biological Process
Cellular response to ionizing radiation Source: UniProtKB
mRNA transcription Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Regulation of cell death Source: UniProtKB
Translational elongation Source: GO_Central
Cellular Location
Isoform 2: Nucleus
More Infomation

Xu, Q., Liu, Y., Wang, S., Wang, J., Liu, L., Xu, Y., & Qin, Y. (2022). Interfering with the expression of EEF1D gene enhances the sensitivity of ovarian cancer cells to cisplatin. BMC cancer, 22(1), 1-15.

Xu, H., Yu, S., Peng, K., Gao, L., Chen, S., Shen, Z., ... & Kang, M. (2021). The role of EEF1D in disease pathogenesis: a narrative review. Annals of Translational Medicine, 9(20).

Hou, Y., Xie, Y., Yang, S., Han, B., Shi, L., Bai, X., ... & Sun, D. (2021). EEF1D facilitates milk lipid synthesis by regulation of PI3K‐Akt signaling in mammals. The FASEB Journal, 35(5), e21455.

Xie, C., Zhou, M., Lin, J., Wu, Z., Ding, S., Luo, J., ... & Song, Y. (2020). EEF1D promotes glioma proliferation, migration, and invasion through EMT and PI3K/Akt pathway. BioMed Research International, 2020.

BİTERGE SÜT, B., & İKİNCİ KELEŞ, A. (2020). Prognostic and Predictive Significance of Eukaryotic Elongation Factor 1D (eEF1D) in Breast Cancer: A Potential Marker of Response to Endocrine Therapy. Oncologie, 22(3).

Ugur Iseri, S. A., Yucesan, E., Tuncer, F. N., Calik, M., Kesim, Y., Altiokka Uzun, G., & Ozbek, U. (2019). Biallelic loss of EEF1D function links heat shock response pathway to autosomal recessive intellectual disability. Journal of Human Genetics, 64(5), 421-426.

Cheng, D. D., Li, S. J., Zhu, B., Zhou, S. M., & Yang, Q. C. (2018). EEF1D overexpression promotes osteosarcoma cell proliferation by facilitating Akt-mTOR and Akt-bad signaling. Journal of Experimental & Clinical Cancer Research, 37(1), 1-10.

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For research use only. Not intended for any clinical use.

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