Mouse Anti-EFHD1 Recombinant Antibody (
7B5) (V2LY-0725-LY57)

Name: Mouse Anti-EFHD1 Recombinant Antibody (7B5)
SKU: V2LY-0725-LY57
Rating: 5 (1 reviews)

See all EFHD1 antibodies

Compare Online Inquiry

Tested Data

Mouse Anti-EFHD1 Recombinant Antibody (7B5) (V2LY-0725-LY57) in IF

IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.

Mouse Anti-EFHD1 Recombinant Antibody (7B5) (V2LY-0725-LY57) in WB

Western blot analysis of 1) Mouse spleen tissue, 2) Rat spleen tissue, diluted at 1:3000.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

7B5

Application

ELISA, WB, IHC, IF

Immunogen

Synthetic Peptide.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:500
IF	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

EF-Hand Domain Family Member D1

Entrez Gene ID

80303

UniProt ID

Q9BUP0

Alternative Names

EF-Hand Domain Family Member D1; Swiprosin-2; EF-Hand Domain-Containing Protein 1; SWS2; EF-Hand Domain-Containing Protein D1; EF Hand Domain Containing 1; MSTP133; MST133; PP3051;

Research Area

Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production (PubMed:26975899).

May play a role in neuronal differentiation (By similarity).

Biological Process

Neuron projection development Source: Ensembl
Regulation of cellular hyperosmotic salinity response Source: UniProtKB

Cellular Location

Mitochondrion inner membrane

Eberhardt, D. R., Lee, S. H., Yin, X., Balynas, A. M., Rekate, E. C., Kraiss, J. N., ... & Chaudhuri, D. (2022). EFHD1 ablation inhibits cardiac mitoflash activation and protects cardiomyocytes from ischemia. Journal of Molecular and Cellular Cardiology, 167, 1-14.

Eberhardt, D. R., Lee, S. H., Yin, X., Balynas, A. M., Rekate, E., Kraiss, J. N., ... & Chaudhuri, D. (2021). EFHD1 ablation reduces cardiac mitoflash activation and protects cardiomyocytes from ischemia. bioRxiv.

Eberhardt, D. R., Yin, X., Balynas, A., Lee, S., Walsh, M., Funai, K., & Chaudhuri, D. (2021, September). Abstract P339: Examining Cardiac Phenotypes Of EF Hand Containing Domain 1 Protein (EFHD1) Knockout Mice. In Circulation Research (Vol. 129, No. Suppl_1, pp. AP339-AP339). Hagerstown, MD: Lippincott Williams & Wilkins.

Mun, S. A., Park, J., Park, K. R., Lee, Y., Kang, J. Y., Park, T., ... & Eom, S. H. (2021). Structural and biochemical characterization of EFhd1/Swiprosin-2, an actin-binding protein in mitochondria. Frontiers in cell and developmental biology, 8, 628222.

Ulisse, V., Dey, S., Rothbard, D. E., Zeevi, E., Gokhman, I., Dadosh, T., ... & Yaron, A. (2020). Regulation of axonal morphogenesis by the mitochondrial protein Efhd1. Life science alliance, 3(7).

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-EFHD1 Recombinant Antibody (
7B5)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-EFHD1 Recombinant Antibody (CAP666) (CAT#: V2LY-0425-LY1497)

Mouse Anti-EFHD1 Recombinant Antibody (CBXS-1028) (CAT#: CBMAB-S3921-CQ)

Mouse Anti-EFHD1 (AA 168-238) Recombinant Antibody (CBFYE-0036) (CAT#: CBMAB-E0068-FY)

Mouse Anti-EFHD1 Recombinant Antibody (CB181) (CAT#: CBMAB-CS181LY)

Mouse Anti-EFHD1 Recombinant Antibody (CB226A) (CAT#: V2LY-0425-LY1496)

Mouse Anti-EFHD1 Recombinant Antibody (CAP666) (CAT#: CBMAB-AP1990LY)

Mouse Anti-EFHD1 Recombinant Antibody (CBT32) (CAT#: V2LY-0625-LY3852)

Mouse Anti-EFHD1 Recombinant Antibody (EC137) (CAT#: V2LY-0624-LY137)

Mouse Anti-EFHD1 Recombinant Antibody (CB181) (CAT#: V2LY-0425-LY1495)

Mouse Anti-EFHD1 Recombinant Antibody (CB226A) (CAT#: CBMAB-CA226LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry