Mouse Anti-EFNA4 Recombinant Antibody (CBFYE-0496) (V2LY-0425-LY1516)

Name: Mouse Anti-EFNA4 Recombinant Antibody (CBFYE-0496)
SKU: V2LY-0425-LY1516
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EFNA4 Recombinant Antibody (CBFYE-0496) (V2LY-0425-LY1516-1) in Immunoblot

Detection of EFNA4-Fc-stimulated ERK activation in OEC-M1 cells. Upper: Immunoblot assay showing total and phosphorylated ERK levels in OEC-M1 cells treated with 0.1 μg/ml EFNA4-Fc for 10, 30, 60, 120, and 360 min. Lower: The phosphorylated ERK level was normalized to that of total ERK. The relative ERK activity was calculated by dividing the level of normalized phosphorylated ERK in OEC-M1 cells treated with EFNA4-Fc (n=2) by that in untreated cells (n=2). ...View More

Chen, Y. L., Yen, Y. C., Jang, C. W., Wang, S. H., Huang, H. T., Chen, C. H., ... & Chen, Y. W. (2021). Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells. Scientific reports, 11(1), 644.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0496

Application

WB, IHC

Immunogen

Recombinant human Ephrin-A4 (Leu26-Gly171).

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1 µg/ml
IHC	5-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

Trehalose & PBS

Preservative

None

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ephrin A4

Entrez Gene ID

1945

UniProt ID

P52798

Research Area

Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. May play a role in the interaction between activated B-lymphocytes and dendritic cells in tonsils.

Biological Process

Axon guidance Source: GO_Central
Cell-cell signaling Source: ProtInc
Ephrin receptor signaling pathway Source: GO_Central

Cellular Location

Isoform 1: Cell membrane
Isoform 2: Secreted

Zhao, X., Chen, Y., Sun, X., He, Z., Wu, T., Wu, C., ... & Liu, X. S. (2022). Oncogenic EFNA4 amplification promotes lung adenocarcinoma lymph node metastasis. Cancers, 14(17), 4226.

Rowe, D. W., Hong, S. H., Zhang, C., Shin, D. G., Adams, D. J., Youngstrom, D. W., ... & Maye, P. (2021). Skeletal screening IMPC/KOMP using μCT and computer automated cryohistology: application to the Efna4 KO mouse line. Bone, 144, 115688.

Lin, J., Zeng, C., Zhang, J., Song, Z., Qi, N., Liu, X., ... & Chen, F. (2021). EFNA4 promotes cell proliferation and tumor metastasis in hepatocellular carcinoma through a PIK3R2/GSK3β/β-catenin positive feedback loop. Molecular Therapy-Nucleic Acids, 25, 328-341.

Garrido‐Laguna, I., Krop, I., Burris III, H. A., Hamilton, E., Braiteh, F., Weise, A. M., ... & Hong, D. S. (2019). First‐in‐human, phase I study of PF‐06647263, an anti‐EFNA4 calicheamicin antibody–drug conjugate, in patients with advanced solid tumors. International journal of cancer, 145(7), 1798-1808.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-EFNA4 Recombinant Antibody (CBFYE-0496) (CAT#: CBMAB-E0849-FY)

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Mouse Anti-EFNA4 Recombinant Antibody (CBFYE-0497) (CAT#: V2LY-0425-LY1517)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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