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Mouse Anti-EFNB3 Recombinant Antibody (CBFYE-0508) (V2LY-0425-LY1527)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBFYE-0508
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 141-310 mapping near the C-terminus of human ephrin-B3.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Ephrin B3
Entrez Gene ID
1949
UniProt ID
Q15768
Research Area
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

(Microbial infection) Acts as a receptor for nipah virus and hendra virus.
Biological Process
Adult walking behavior Source: Ensembl
Axon choice point recognition Source: Ensembl
Axon guidance Source: GO_Central
Cell-cell signaling Source: ProtInc
Ephrin receptor signaling pathway Source: MGI
Negative regulation of axonogenesis Source: ARUK-UCL
Nervous system development Source: ProtInc
T cell costimulation Source: Ensembl
Trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmission Source: Ensembl
Cellular Location
Membrane
Topology
Extracellular: 28-226
Helical: 227-247
Cytoplasmic: 248-340

Wu, T., Zhang, B. Q., Raelson, J., Yao, Y. M., Wu, H. D., Xu, Z. X., ... & Hu, S. J. (2018). Analysis of the association of EPHB6, EFNB1 and EFNB3 variants with hypertension risks in males with hypogonadism. Scientific reports, 8(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-EFNB3 Recombinant Antibody (CBFYE-0508) (CAT#: CBMAB-E0864-FY)

Related Products

Mouse Anti-EFNB3 Recombinant Antibody (CBFYE-0508) (CAT#: CBMAB-E0864-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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