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Mouse Recombinant EFNB3 protein, His Tag (V2LY-0526-LY8268)

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Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host
HEK293 Cells
Protein Species
Mouse
Tag
His Tag
Protein Construction
This product is Mouse Recombinant EFNB3 protein, His Tag consist of Amino Acid: 1-227 and predicts a molecular mass of 23.5 kDa.
Molecule Mass
23.5 kDa
Sequence
Amino Acid: 1-227
Species
Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by SDS-PAGE.
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Ephrin B3
Research Area
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).

(Microbial infection) Acts as a receptor for nipah virus and hendra virus.
Biological Process
Adult walking behavior Source: Ensembl
Axon choice point recognition Source: Ensembl
Axon guidance Source: GO_Central
Cell-cell signaling Source: ProtInc
Ephrin receptor signaling pathway Source: MGI
Negative regulation of axonogenesis Source: ARUK-UCL
Nervous system development Source: ProtInc
T cell costimulation Source: Ensembl
Trans-synaptic signaling by trans-synaptic complex, modulating synaptic transmission Source: Ensembl
Cellular Location
Membrane
Topology
Extracellular: 28-226
Helical: 227-247
Cytoplasmic: 248-340

Wu, T., Zhang, B. Q., Raelson, J., Yao, Y. M., Wu, H. D., Xu, Z. X., ... & Hu, S. J. (2018). Analysis of the association of EPHB6, EFNB1 and EFNB3 variants with hypertension risks in males with hypogonadism. Scientific reports, 8(1), 1-9.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant EFNB3 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8267)

Rat Recombinant EFNB3 protein, hFc Tag (CAT#: V2LY-0526-LY9363)

Rat Recombinant EFNB3 protein, His Tag (CAT#: V2LY-0526-LY9364)

Related Products

Mouse Recombinant EFNB3 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY8267)

Mouse Anti-EFNB3 Recombinant Antibody (CBFYE-0508) (CAT#: V2LY-0425-LY1527)

Mouse Anti-EFNB3 Recombinant Antibody (CBFYE-0508) (CAT#: CBMAB-E0864-FY)

Rat Recombinant EFNB3 protein, hFc Tag (CAT#: V2LY-0526-LY9363)

Rat Recombinant EFNB3 protein, His Tag (CAT#: V2LY-0526-LY9364)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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