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Mouse Anti-EHD2 Recombinant Antibody (CBFYE-0053) (V2LY-0425-LY1596)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYE-0053
Antibody Isotype
IgG3, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 414-441 within an internal region of human EHD2.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG3, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
EH-domain containing 2
Entrez Gene ID
Human30846
Mouse259300
Rat361512
UniProt ID
HumanQ9NZN4
MouseQ8BH64
RatQ4V8H8
Research Area
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity).

Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed:17233914).

Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity).

Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (PubMed:25588833).
Biological Process
Cilium assembly Source: GO_Central
Cortical actin cytoskeleton organization Source: Ensembl
Endocytic recycling Source: UniProtKB
Endocytosis Source: GO_Central
Endosomal transport Source: GO_Central
Plasma membrane tubulation Source: UniProtKB
Positive regulation of endocytic recycling Source: UniProtKB
Positive regulation of myoblast fusion Source: UniProtKB
Protein localization to plasma membrane Source: UniProtKB
Cellular Location
Cytosol; Endosome membrane; Cell membrane; Caveola. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes.
More Infomation

Jones, T., Naslavsky, N., & Caplan, S. (2022). Differential requirements for the Eps15 homology Domain Proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis. Traffic.

Wei, S., Shao, J., Wang, J., Zhao, T., Liu, J., Shen, X., ... & Wang, G. (2022). EHD2 inhibits the invasive ability of lung adenocarcinoma and improves the prognosis of patients. Journal of thoracic disease, 14(7), 2652.

Fryklund, C., Morén, B., Shah, S., Grossi, M., Degerman, E., Matthaeus, C., & Stenkula, K. G. (2021). EH Domain-Containing 2 Deficiency Restricts Adipose Tissue Expansion and Impairs Lipolysis in Primary Inguinal Adipocytes. Frontiers in Physiology, 12.

Guan, C., Lu, C., Xiao, M., & Chen, W. (2021). EHD2 overexpression suppresses the proliferation, migration, and invasion in human colon cancer. Cancer Investigation, 39(4), 297-309.

Liu, C., Liu, S., Wang, L., Wang, Y., Li, Y., & Cui, Y. (2019). Effect of EH domain containing protein 2 on the biological behavior of clear cell renal cell carcinoma. Human & Experimental Toxicology, 38(8), 927-937.

Morén, B., Hansson, B., Negoita, F., Fryklund, C., Lundmark, R., Göransson, O., & Stenkula, K. G. (2019). EHD2 regulates adipocyte function and is enriched at cell surface–associated lipid droplets in primary human adipocytes. Molecular biology of the cell, 30(10), 1147-1159.

Matthäus, C., Lahmann, I., Kunz, S., Jonas, W., Melo, A. A., Lehmann, M., ... & Daumke, O. (2019). EHD2-mediated restriction of caveolar dynamics regulates cellular lipid uptake. bioRxiv, 511709.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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