Mouse Anti-EHD2 Recombinant Antibody (CBFYE-0615) (CBMAB-E1016-FY)

Name: Mouse Anti-EHD2 Recombinant Antibody (CBFYE-0615)
SKU: CBMAB-E1016-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0615

Application

WB, IP, IF, ELISA

Immunogen

Recombinant human EHD2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

EH-domain containing 2

Introduction

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Entrez Gene ID

Human	30846
Mouse	259300
Rat	361512

UniProt ID

Human	Q9NZN4
Mouse	Q8BH64
Rat	Q4V8H8

Alternative Names

EH Domain Containing 2; PAST Homolog 2; PAST2; EH Domain-Containing Protein 2

Research Area

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity).

Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed:17233914).

Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity).

Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (PubMed:25588833).

Biological Process

Cilium assembly Source: GO_Central
Cortical actin cytoskeleton organization Source: Ensembl
Endocytic recycling Source: UniProtKB
Endocytosis Source: GO_Central
Endosomal transport Source: GO_Central
Plasma membrane tubulation Source: UniProtKB
Positive regulation of endocytic recycling Source: UniProtKB
Positive regulation of myoblast fusion Source: UniProtKB
Protein localization to plasma membrane Source: UniProtKB

Cellular Location

Cytosol; Endosome membrane; Cell membrane; Caveola. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes.

Jones, T., Naslavsky, N., & Caplan, S. (2022). Differential requirements for the Eps15 homology Domain Proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis. Traffic.

Wei, S., Shao, J., Wang, J., Zhao, T., Liu, J., Shen, X., ... & Wang, G. (2022). EHD2 inhibits the invasive ability of lung adenocarcinoma and improves the prognosis of patients. Journal of thoracic disease, 14(7), 2652.

Fryklund, C., Morén, B., Shah, S., Grossi, M., Degerman, E., Matthaeus, C., & Stenkula, K. G. (2021). EH Domain-Containing 2 Deficiency Restricts Adipose Tissue Expansion and Impairs Lipolysis in Primary Inguinal Adipocytes. Frontiers in Physiology, 12.

Guan, C., Lu, C., Xiao, M., & Chen, W. (2021). EHD2 overexpression suppresses the proliferation, migration, and invasion in human colon cancer. Cancer Investigation, 39(4), 297-309.

Liu, C., Liu, S., Wang, L., Wang, Y., Li, Y., & Cui, Y. (2019). Effect of EH domain containing protein 2 on the biological behavior of clear cell renal cell carcinoma. Human & Experimental Toxicology, 38(8), 927-937.

Morén, B., Hansson, B., Negoita, F., Fryklund, C., Lundmark, R., Göransson, O., & Stenkula, K. G. (2019). EHD2 regulates adipocyte function and is enriched at cell surface–associated lipid droplets in primary human adipocytes. Molecular biology of the cell, 30(10), 1147-1159.

Matthäus, C., Lahmann, I., Kunz, S., Jonas, W., Melo, A. A., Lehmann, M., ... & Daumke, O. (2019). EHD2-mediated restriction of caveolar dynamics regulates cellular lipid uptake. bioRxiv, 511709.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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