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Mouse Anti-EHD2 Recombinant Antibody (CBFYE-0614) (CBMAB-E1014-FY)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYE-0614
Antibody Isotype
IgG1, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
EHD2 (aa365-427) partial recombinant protein.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
None
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 365-428

Target

Full Name
EH-domain containing 2
Introduction
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.
Entrez Gene ID
Human30846
Mouse259300
Rat361512
UniProt ID
HumanQ9NZN4
MouseQ8BH64
RatQ4V8H8
Alternative Names
EH Domain Containing 2; PAST Homolog 2; PAST2; EH Domain-Containing Protein 2
Research Area
ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (By similarity).

Plays a role in membrane trafficking between the plasma membrane and endosomes (PubMed:17233914).

Important for the internalization of GLUT4. Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (By similarity).

Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (PubMed:25588833).
Biological Process
Cilium assembly Source: GO_Central
Cortical actin cytoskeleton organization Source: Ensembl
Endocytic recycling Source: UniProtKB
Endocytosis Source: GO_Central
Endosomal transport Source: GO_Central
Plasma membrane tubulation Source: UniProtKB
Positive regulation of endocytic recycling Source: UniProtKB
Positive regulation of myoblast fusion Source: UniProtKB
Protein localization to plasma membrane Source: UniProtKB
Cellular Location
Cytosol; Endosome membrane; Cell membrane; Caveola. Colocalizes with GLUT4 in intracellular tubulovesicular structures that are associated with cortical F-actin. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes.
More Infomation

Jones, T., Naslavsky, N., & Caplan, S. (2022). Differential requirements for the Eps15 homology Domain Proteins EHD4 and EHD2 in the regulation of mammalian ciliogenesis. Traffic.

Wei, S., Shao, J., Wang, J., Zhao, T., Liu, J., Shen, X., ... & Wang, G. (2022). EHD2 inhibits the invasive ability of lung adenocarcinoma and improves the prognosis of patients. Journal of thoracic disease, 14(7), 2652.

Fryklund, C., Morén, B., Shah, S., Grossi, M., Degerman, E., Matthaeus, C., & Stenkula, K. G. (2021). EH Domain-Containing 2 Deficiency Restricts Adipose Tissue Expansion and Impairs Lipolysis in Primary Inguinal Adipocytes. Frontiers in Physiology, 12.

Guan, C., Lu, C., Xiao, M., & Chen, W. (2021). EHD2 overexpression suppresses the proliferation, migration, and invasion in human colon cancer. Cancer Investigation, 39(4), 297-309.

Liu, C., Liu, S., Wang, L., Wang, Y., Li, Y., & Cui, Y. (2019). Effect of EH domain containing protein 2 on the biological behavior of clear cell renal cell carcinoma. Human & Experimental Toxicology, 38(8), 927-937.

Morén, B., Hansson, B., Negoita, F., Fryklund, C., Lundmark, R., Göransson, O., & Stenkula, K. G. (2019). EHD2 regulates adipocyte function and is enriched at cell surface–associated lipid droplets in primary human adipocytes. Molecular biology of the cell, 30(10), 1147-1159.

Matthäus, C., Lahmann, I., Kunz, S., Jonas, W., Melo, A. A., Lehmann, M., ... & Daumke, O. (2019). EHD2-mediated restriction of caveolar dynamics regulates cellular lipid uptake. bioRxiv, 511709.

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For research use only. Not intended for any clinical use.

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