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Mouse Anti-EID2 Recombinant Antibody (CBFYE-0631) (V2LY-0425-LY1623)


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Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
CBFYE-0631
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 110-138 within an internal region of EID-2.
Host Species
Mouse
Specificity
Mouse, Rat, Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
EP300 Interacting Inhibitor Of Differentiation 2
Entrez Gene ID
Human163126
Mouse386655
Rat685691
UniProt ID
HumanQ8N6I1
MouseQ6X7S9
RatD4ACB5
Research Area
Interacts with EP300 and acts as a repressor of MYOD-dependent transcription and muscle differentiation. Inhibits EP300 histone acetyltransferase activity. Acts as a repressor of TGFB/SMAD transcriptional responses. May act as a repressor of the TGFB/SMAD3-dependent signaling by selectively blocking formation of TGFB-induced SMAD3-SMAD4 complex.
Biological Process
Cell differentiation Source: UniProtKB-KW
Muscle organ development Source: UniProtKB-KW
Negative regulation of transcription, DNA-templated Source: HGNC-UCL
Negative regulation of transcription by RNA polymerase II Source: InterPro
Negative regulation of transforming growth factor beta receptor signaling pathway Source: HGNC-UCL
Regulation of cell population proliferation Source: HGNC-UCL
Regulation of transforming growth factor beta receptor signaling pathway Source: HGNC-UCL
SMAD protein complex assembly Source: HGNC-UCL
Transforming growth factor beta receptor complex assembly Source: HGNC-UCL
Cellular Location
Nucleus
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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