Mouse Anti-EID2 Recombinant Antibody (CBFYE-0631) (V2LY-0425-LY1623)

Name: Mouse Anti-EID2 Recombinant Antibody (CBFYE-0631)
SKU: V2LY-0425-LY1623
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0631

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 110-138 within an internal region of EID-2.

Host Species

Mouse

Specificity

Mouse, Rat, Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

EP300 Interacting Inhibitor Of Differentiation 2

Entrez Gene ID

Human	163126
Mouse	386655
Rat	685691

UniProt ID

Human	Q8N6I1
Mouse	Q6X7S9
Rat	D4ACB5

Research Area

Interacts with EP300 and acts as a repressor of MYOD-dependent transcription and muscle differentiation. Inhibits EP300 histone acetyltransferase activity. Acts as a repressor of TGFB/SMAD transcriptional responses. May act as a repressor of the TGFB/SMAD3-dependent signaling by selectively blocking formation of TGFB-induced SMAD3-SMAD4 complex.

Biological Process

Cell differentiation Source: UniProtKB-KW
Muscle organ development Source: UniProtKB-KW
Negative regulation of transcription, DNA-templated Source: HGNC-UCL
Negative regulation of transcription by RNA polymerase II Source: InterPro
Negative regulation of transforming growth factor beta receptor signaling pathway Source: HGNC-UCL
Regulation of cell population proliferation Source: HGNC-UCL
Regulation of transforming growth factor beta receptor signaling pathway Source: HGNC-UCL
SMAD protein complex assembly Source: HGNC-UCL
Transforming growth factor beta receptor complex assembly Source: HGNC-UCL

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-EID2 Recombinant Antibody (CBFYE-0631) (CAT#: CBMAB-E1037-FY)

Related Products

Mouse Anti-EID2 Recombinant Antibody (CBFYE-0631) (CAT#: CBMAB-E1037-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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