Mouse Anti-EIF3K Recombinant Antibody (CBFYE-0690) (V2LY-0425-LY1705)

Name: Mouse Anti-EIF3K Recombinant Antibody (CBFYE-0690)
SKU: V2LY-0425-LY1705
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog, Cattle, Pig

Clone

CBFYE-0690

Antibody Isotype

IgG2a, κ

Application

ELISA, IF, IP, WB

Basic Information

Immunogen

Full length of human Eif3k.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Horse, Dog, Cattle, Pig

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Eukaryotic Translation Initiation Factor 3 Subunit K

Entrez Gene ID

27335

UniProt ID

Q9UBQ5

Research Area

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815).

The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632).

The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Biological Process

Formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
Regulation of translational initiation Source: InterPro
Translational initiation Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm

More Infomation

References

Chen, Y., Cao, B., Zheng, W., Sun, Y., & Xu, T. (2022). eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish. Journal of Biological Chemistry, 298(3).

Duan, H., Zhang, S., Zarai, Y., Öllinger, R., Wu, Y., Sun, L., ... & Wolf, D. A. (2022). eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content. bioRxiv.

Lin, L., Cao, J., Du, A., An, Q., Chen, X., Yuan, S., ... & Norvienyeku, J. (2021). eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae. Frontiers in plant science, 12, 748120.

Lin, L., Du, A., An, Q., Chen, X., Yuan, S., Batool, W., ... & Norvienyeku, J. (2021). Corrigendum: eIF3k domain-containing protein regulates conidiogenesis, appressorium turgor, virulence, stress tolerance, physiological and pathogenic development of Magnaporthe oryzae Oryzae. Frontiers in Plant Science, 2790.

Yao, L., Xu, B., & Li, X. (2020). Neisseria gonorrhoeae-induced salpingitis is targeted by circular RNA EIF3K via miR-139-5p and regulating MAPK/NF-κB signaling pathway to promotes apoptosis and autophagy bacterial cells. Microbial pathogenesis, 142, 104051.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Mouse Anti-EIF3K Recombinant Antibody (CBFYE-0690)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

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SDS
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