Human Recombinant EIF3K protein, GST Tag (V2LY-0526-LY3823)

Name: Human Recombinant EIF3K protein, GST Tag
SKU: V2LY-0526-LY3823
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant EIF3K protein, GST Tag consist of Amino Acid: 2-218 and predicts a molecular mass of 52.8 kDa.

Molecule Mass

52.8 kDa

Sequence

Amino Acid: 2-218

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Eukaryotic Translation Initiation Factor 3 Subunit K

Research Area

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815).

The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632).

The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Biological Process

Formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
Regulation of translational initiation Source: InterPro
Translational initiation Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm

Chen, Y., Cao, B., Zheng, W., Sun, Y., & Xu, T. (2022). eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish. Journal of Biological Chemistry, 298(3).

Duan, H., Zhang, S., Zarai, Y., Öllinger, R., Wu, Y., Sun, L., ... & Wolf, D. A. (2022). eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content. bioRxiv.

Lin, L., Cao, J., Du, A., An, Q., Chen, X., Yuan, S., ... & Norvienyeku, J. (2021). eIF3k Domain-Containing Protein Regulates Conidiogenesis, Appressorium Turgor, Virulence, Stress Tolerance, and Physiological and Pathogenic Development of Magnaporthe oryzae Oryzae. Frontiers in plant science, 12, 748120.

Lin, L., Du, A., An, Q., Chen, X., Yuan, S., Batool, W., ... & Norvienyeku, J. (2021). Corrigendum: eIF3k domain-containing protein regulates conidiogenesis, appressorium turgor, virulence, stress tolerance, physiological and pathogenic development of Magnaporthe oryzae Oryzae. Frontiers in Plant Science, 2790.

Yao, L., Xu, B., & Li, X. (2020). Neisseria gonorrhoeae-induced salpingitis is targeted by circular RNA EIF3K via miR-139-5p and regulating MAPK/NF-κB signaling pathway to promotes apoptosis and autophagy bacterial cells. Microbial pathogenesis, 142, 104051.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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