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Mouse Anti-EIF3H Recombinant Antibody (3B12) (CBMAB-A2533-LY)

The product is antibody recognizes EIF3S3. The antibody 3B12 immunoassay techniques such as: WB, ELISA.
See all EIF3H antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3B12
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
EIF3S3 (NP_003747, 152 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 3 Subunit H
Entrez Gene ID
8667
UniProt ID
O15372
Alternative Names
EIF3S3; MGC102958; eIF3-gamma; eIF3-p40
Research Area
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815).

The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632).

The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Biological Process
Formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
Negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
Regulation of translational initiation Source: UniProtKB
Translational initiation Source: UniProtKB
Cellular Location
Cytoplasm

Zheng, R., Zhang, K., Tan, S., Gao, F., Zhang, Y., Xu, W., ... & Wang, M. (2022). Exosomal circLPAR1 functions in colorectal cancer diagnosis and tumorigenesis through suppressing BRD4 via METTL3–eIF3h interaction. Molecular cancer, 21(1), 1-14.

Zhu, Y. D., Ba, H., Chen, J., Zhang, M., & Li, P. (2021). Celastrus orbiculatus Extract Reduces Stemness of Gastric Cancer Stem Cells by Targeting PDCD4 and EIF3H. Integrative Cancer Therapies, 20, 15347354211058168.

Zheng, W., Li, Y., Su, Z., Zhang, J., Shi, F., & Liang, W. (2021). EIF3H knockdown inhibits malignant melanoma through regulating cell proliferation, apoptosis and cell cycle. Experimental Cell Research, 402(1), 112488.

Guo, X., Zhu, R., Luo, A., Zhou, H., Ding, F., Yang, H., & Liu, Z. (2020). EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability. Journal of Experimental & Clinical Cancer Research, 39(1), 1-15.

Zhou, Z., Zhou, H., Ponzoni, L., Luo, A., Zhu, R., He, M., ... & Wan, Y. (2020). EIF3H Orchestrates Hippo Pathway–Mediated Oncogenesis via Catalytic Control of YAP StabilityEIF3H Regulates Hippo–YAP Pathway in Tumor Metastasis. Cancer research, 80(12), 2550-2563.

Hu, Y., Wei, X., Lv, Y., Xie, X., Yang, L., He, J., ... & Liu, Z. (2020). EIF3H interacts with PDCD4 enhancing lung adenocarcinoma cell metastasis. American journal of cancer research, 10(1), 179.

Yu, G., Liao, J., Wu, J., Ding, J., & Zhang, L. (2018). The proliferation of colorectal cancer cells is suppressed by silencing of EIF3H. Bioscience, biotechnology, and biochemistry, 82(10), 1694-1701.

Choe, J., Lin, S., Zhang, W., Liu, Q., Wang, L., Ramirez-Moya, J., ... & Gregory, R. I. (2018). mRNA circularization by METTL3–eIF3h enhances translation and promotes oncogenesis. Nature, 561(7724), 556-560.

Wang, X., Wang, H., Zhao, S., Sun, P., Wen, D., Liu, T., ... & Ma, Z. (2018). Eukaryotic translation initiation factor EIF3H potentiates gastric carcinoma cell proliferation. Tissue and Cell, 53, 23-29.

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For research use only. Not intended for any clinical use.

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